Integrated test reaction plate of five indicators of prenatal and postnatal care and kit

A detection kit and integrated detection technology, applied in the field of in vitro clinical testing and biochips, can solve the problems of laboratory personnel's health threat, complex composition, large sample size, etc., to improve detection speed and efficiency, simple production process, and monitoring results. stable effect

Inactive Publication Date: 2010-07-07
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional ELISA detects antibodies, often detecting IgM and IgG antibodies, which can judge recent infection (IgM index) and detect the antibody (immunity) level of the population (IgG index); but if you want toxoplasma gondii (Toxopasma), Rubella virus (Rubella.Virus), cytomegalovirus (Cytomegalo.Virus), herpes simplex type I and herpes simplex type II (Herpes.Virus) 5 pathogens are detected, and more than a dozen kits are needed to detect all IgG and IgM After the detection is completed, only one indicator can be detected for each reaction, which is slow, inefficient, expensive, and requires a large amount of samples
Moreover, the antigens in the kits currently used in the market are all crudely extracted antigens from pathogen cul

Method used

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  • Integrated test reaction plate of five indicators of prenatal and postnatal care and kit
  • Integrated test reaction plate of five indicators of prenatal and postnatal care and kit
  • Integrated test reaction plate of five indicators of prenatal and postnatal care and kit

Examples

Experimental program
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Example Embodiment

[0039] Example 1:

[0040] Preparation of an integrated detection reaction plate for five indexes of prenatal and postnatal care

[0041] The main steps are: (1) prepare the substrate nitrocellulose membrane; (2) design the chip, determine the arrangement of the dot matrix and the spotting position; (3) aspirate the antibody from the 384-well plate and spray the spot on the nitrocellulose On the membrane; (4) Assemble the nitrocellulose membrane, and carry out sealing, drying, packaging and storage.

[0042] The method for preparing the reaction plate for detecting the five indexes of prenatal and postnatal care specifically includes the following steps:

[0043] 1. Determination of spot antigens related to five indicators of prenatal and postnatal care testing:

[0044] Toxoplasma gondii Antigen (Strain RH), Rubella Antigen (HPV-77), Cytomegalovirus Ag (AD169), Herpes Simplex Virus I (HSV1) and Herpes Simplex Virus II (HSV2 whole virus antigens) purchased from BioDesign.

[0045] Deter...

Example Embodiment

[0053] Example 2:

[0054] Preparation of an integrated test kit for five indexes of prenatal and postnatal care

[0055] Including the five indicators of prenatal and postnatal care integrated test reaction plate, negative control substance, positive control substance I, II, sample diluent, enzyme label working solution I, II, concentrated washing solution, test solution A, B composition; each part is bottled, Fix it in the box with a paper template (e.g. image 3 ), not limited to the placement method, it is used when testing. The following is the preparation method of each part:

[0056] 1. The eugenics and eugenics detection protein chip is a 6×8-well plate with a nitrocellulose membrane as the base. See ZL032298900 for the assembly method. See the appearance after completion figure 1 .

[0057] 2. One bottle of negative control 5 is provided, each bottle is 0.4ml;

[0058] It is formulated with 3% BSA and 0.05% preservative (ProclinTM 300) in phosphate buffer (pH 7.4). Provide ...

Example Embodiment

[0070] Example 3:

[0071] Application and detection of integrated test kit for five indicators of prenatal and postnatal care

[0072] The application of the integrated detection kit for detecting the five indexes of prenatal and postnatal care of the present invention mainly adopts the antigen-antibody-enzyme-labeled secondary antibody method to detect the five prenatal and postnatal care antibodies in human blood. The method is to add human serum to the surface of the chip, and the antibodies in the sample are respectively bound to the corresponding antigens immobilized on the chip. After drying, add the enzyme-labeled secondary antibody to react to wash off the unbound secondary antibody. After adding the detection solution 1 Minutes, scan and collect the signal, the signal intensity of the sample point is proportional to the concentration of the antibody in the serum.

[0073] In the application of the protein chip kit for detecting the five items of prenatal and postnatal care...

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Abstract

In order to increase the clinical test efficiency of TORCH five indicators and reduce the test time and cost, the invention provides an integrated reaction plate for parallel test of 5 items of viral infection related to prenatal and postnatal care and a kit comprising same. The integrated test reaction plate comprises a substrate and point-like indicators distributed on the substrate in an array type, wherein the point-like indicators comprise toxoplasma gondii antigen (Strain RH), rubella virus antigen (HPV-77), giant cell antigen (AD 169), herpes simplex antigen type I (HSV1) and herpes simplex antigen type I I (HSV2). The integrated test reaction plate is used for testing 5 items of prenatal and postnatal care pathogens antibodies and has the characteristics of less samples, time saving, labor saving and high accuracy.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing and biochip technology, and in particular relates to an integrated reaction plate and a kit for detecting five virus infection indicators related to prenatal and postnatal care. Background technique [0002] The word "TORCH" was created by Nahmias in 1971. It is a combination of English name prefixes for a group of pathogenic microorganisms. TO stands for Toxoplasma gondi (Toxo), and R stands for Rubella Virus (RV). , C stands for cytomegalovirus (Cytomegalovirus, CMV), H stands for Herpes Simplex Virus I and II (HSVI, HSV2), and the English meaning of TORCH is "torch, torch". The purpose of condensing such pathogenic microorganisms into the word "TORCH" is to suggest its importance, in order to attract the attention of obstetricians and pediatricians. [0003] These five pathogens have a wide range of infections and are very harmful. Pregnant women are prone to primary infections due to ...

Claims

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Application Information

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IPC IPC(8): G01N33/571G01N33/543
Inventor 穆海东汪宁梅穆宇豪刘纲顾蓉
Owner 上海裕隆生物科技有限公司
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