Thecodiplosis japonensis larva separating method and application thereof

A separation method, the technology of sheath galls, which is applied in the field of separation of overwintering larvae of pine needle sheath gall mosquitoes, can solve the problems of high missed detection rate, labor-intensive, time-consuming, and low efficiency, so as to reduce the missed detection rate, fast and efficient separation, and save manpower and material costs Effect

Active Publication Date: 2019-05-31
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventors found that at present, the method of picking up the soil by hand (bring the collected soil sample back to the laboratory, spread it out little by little with tweezers or insect needles to find the overwintering larvae inside) is used to determine the number of overwintering larvae in the soil sample, which is labor-intensive. Time-consuming and inefficient, and there is a high rate of missed detection

Method used

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  • Thecodiplosis japonensis larva separating method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Take a soil sample of 1000g, first use a 1# sampling sieve (diameter 20cm, height 6cm, aperture 4mm, 304 stainless steel material) to sieve out the litter, fallen leaves and large gravel in the soil sample, and lay a black polymer under the sampling sieve. Ethylene plastic film (0.5m x 0.5m, thickness 0.05-0.12mm, preferably 0.1mm) is used to receive the worm-infested soil samples sieved for future use.

[0047]2. Transfer the worm soil sample screened out by the 1# sampling sieve to the 2# sampling sieve (diameter 30cm, height 6cm, aperture 1.6mm, 304 stainless steel material) to sieve out the medium-sized gravel in the soil sample, and use the sampling sieve Lay a black polyethylene plastic film (0.5m * 0.5m, thickness 0.05-0.12mm, preferably 0.1mm) to receive the wormy soil samples that are sieved down for subsequent use.

[0048] 3. Transfer the worm soil sample screened out by 2# sampling sieve to 3# sampling sieve (diameter 30cm, height 6cm, aperture 0.45mm, pr...

Embodiment 2

[0052] 1. Take a soil sample of 1500g, first use a 1# sampling sieve (diameter 20cm, height 6cm, aperture 5mm, 304 stainless steel material) to sieve out the litter, fallen leaves and large gravel in the soil sample, and lay a black polymer under the sampling sieve. Ethylene plastic film (0.5m×0.5m, thickness 0.05-0.12mm, preferably 0.1mm) is used to receive the worm-infected soil samples sieved for future use.

[0053] 2. Transfer the worm-infected soil sample screened out by the 1# sampling sieve to the 2# sampling sieve (diameter 30cm, height 6cm, aperture 2mm, 304 stainless steel material) to sieve out the medium-sized gravel in the soil sample, and put it under the sampling sieve. Spread a black polyethylene plastic film (0.5m * 0.5m, thickness 0.05-0.12mm, preferably 0.1mm) to receive the wormy soil samples that are sieved down for subsequent use.

[0054] 3. Transfer the worm soil sample screened out by 2# sampling sieve to 3# sampling sieve (diameter 30cm, height 6cm, ...

Embodiment 3

[0058] 1. Take a soil sample of 1200g, first use a 1# sampling sieve (diameter 20cm, height 6cm, aperture 5mm, 304 stainless steel material) to sieve out the litter, fallen leaves and large gravel in the soil sample, and lay a black polymer under the sampling sieve. Ethylene plastic film (0.5m×0.5m, thickness 0.05-0.12mm, preferably 0.1mm) is used to receive the worm-infected soil samples sieved for future use.

[0059] 2. Transfer the worm soil sample screened out by the 1# sampling sieve to the 2# sampling sieve (diameter 30cm, height 6cm, aperture 1.6mm, 304 stainless steel material) to sieve out the medium-sized gravel in the soil sample, and use the sampling sieve Lay a black polyethylene plastic film (0.5m * 0.5m, thickness 0.05-0.12mm, preferably 0.1mm) to receive the wormy soil samples that are sieved down for subsequent use.

[0060] 3. Transfer the worm soil sample screened out by 2# sampling sieve to 3# sampling sieve (diameter 30cm, height 6cm, aperture 0.45mm, pre...

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Abstract

The invention provides a thecodiplosis japonensis larva separating method and application thereof. The separating method includes the steps of taking a to-be-detected soil sample to be placed in a first sample separating sieve to screen out dry branches, fallen leaves and large gravels in the soil sample, placing the screened soil sample in a second sample separating sieve to screen out middle-size gravels in the soil sample, placing the screened soil sample in a third sample separating sieve to screen out fine soil in the soil, conducting water bath vibrating treatment on the third sample separating sieve filled with the soil sample, placing the third sample separating sieve subjected to he water bath vibrating treatment in clean water, conducting stirring and removing floating objects, and then scooping up larvae with a strainer repeatedly until the larvae in the third sample separating sieve are completely scooped up. By means of the separating method, the thecodiplosis japonensis larvae can be rapidly separated from the soil and collected, the method is simple and easy to implement, no complicated devices are needed, labor and material cost is greatly saved, the detection speedand efficiency are remarkably improved, the detection omission rate is remarkably reduced, and the method has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of pest monitoring and prevention, and in particular relates to a method for separating overwintering larvae of pine needle sheath gall midges and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The codiplosis Japonensis Uchida&Inouye belongs to Diplera, Cecidomyidae, Thecodiplosis. important invasive pests. The larvae hide in the base of the pine needles and suck the juice of the leaf base, causing the base of the needles to expand and form galls, the growth stagnates, and gradually withers and falls off. After 2-3 years of continuous damage, the vigor of the host tree will be s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033B07B9/00
Inventor 武海卫段春华亓玉昆刘焕秀刘慇陈汝敏陈素伟潘涛高尚坤季英超周成刚
Owner SHANDONG FOREST SCI RES INST
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