Tenebrio molitor larva enzymatic hydrolysate and preparation method thereof

An enzymatic hydrolyzate and barley worm technology, which is applied in the food field, can solve the problems of large market price fluctuation, low protein content and high price, and achieve the effects of improving the overall aroma intensity, high protein content and low cost.

Active Publication Date: 2014-08-06
TIANJIN CHUNFA BIO TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of high price, large market price fluctuations and low protein content of the essence raw material protein enzymatic hydrolyzate in the prior art, and provide a barley worm larva enzymolyzate for preparing essence and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of barley worm larva enzymatic hydrolyzate, comprising the steps of:

[0033] Put 400g of washed and crushed barley worm larvae and 100g of distilled water into the reactor, add 0.8g of papain, stir for 10min, adjust the pH value to 6.0~7.0, heat up, the reaction temperature is 65℃ for 1h, and the enzyme After the digestion, heat up to 100°C to inactivate the enzyme for 20 minutes, then cool down to 50°C after the end of the enzyme inactivation, let it stand at room temperature for 30 minutes, filter through a 40-mesh sieve, filter out the residue, and collect the filtrate to obtain the enzymatic hydrolyzate of barley worm larvae.

Embodiment 2

[0035] A preparation method of barley worm larva enzymatic hydrolyzate, comprising the steps of:

[0036] Wash and crush 350g of barley worm larvae, put 150g of distilled water in the reactor, add a mixture of 0.4g of trypsin and 0.2g of neutral protease, stir for 5min, adjust the pH value to 6.5~7.0, heat up, and the reaction temperature is Enzymolysis at 70°C for 0.5h. After enzymolysis, heat up to 90°C to inactivate the enzyme for 20 minutes. After inactivation, cool down to 45°C. Leave it at room temperature for 20 minutes. Filter through a 40-mesh sieve to remove the residue and collect the filtrate to obtain the barley worm Larval hydrolyzate.

Embodiment 3

[0038] A method for preparing a pork reaction essence obtained by applying the enzymatic hydrolysis solution of barley worm larvae, comprising the following steps:

[0039] Get pork enzymolysis solution: 50g, barley worm larva enzymolysis solution prepared in Example 1: 30g, refined lard: 6g, cysteine ​​hydrochloride: 0.5g, glycine: 0.5g, proline 0.5g , leucine 0.5g, methionine: 0.3g, glucose: 6g, VB1: 0.5g, I+G: 0.5g, yeast extract: 1g, HVP1g, green onion mud 1g, ginger mud 1g join reactor, stir After fully mixing for 15 minutes, heat up and control the thermal reaction temperature at 100°C for 1.5h. After the reaction is completed, lower it to 50°C. After standing at room temperature for 25 minutes, filter it with a 40-mesh sieve. The filtrate is pork reaction essence.

[0040] Wherein the preparation method of pork enzymolysis solution:

[0041] Put 350g of washed and minced pork and 150g of distilled water into the reactor, add 0.5g of papain, stir for 15min, adjust the p...

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Abstract

The invention relates to tenebrio molitor larva enzymatic hydrolysate and a preparation method thereof. The tenebrio molitor larva enzymatic hydrolysate is prepared by the following raw materials of tenebrio molitor larva, proteinase and distilled water, wherein the weight part ratio of the tenebrio molitor larva to the distilled water is 70-90 parts to 10-30 parts, and the additive amount of the proteinase is 0.05-0.2 percent of the total weight of the tenebrio molitor larva and the distilled water. The tenebrio molitor larva is used as enzymolysis protein, which has high protein content compared with poultry meat, and low cost; protein enzymatic hydrolysis liquid of amino acid and peptide with high concentration can be obtained; and when the tenebrio molitor larva enzymatic hydrolysate is applied in heat reaction, more flavored compounds can be generated.

Description

technical field [0001] The invention relates to the technical field of food, in particular to a barley worm larva enzymatic hydrolyzate and a preparation method thereof. Background technique [0002] With the change of food structure and the vigorous development of tourism, the convenience food industry has also developed rapidly. Returning to nature and seeking green food has become the trend of the times for the development of the convenience industry. Essence is one of the rapidly developing flavors in recent years. It is widely used in flavoring and flavoring of convenience food, puffed food and meat products. High requirements, the Maillard reaction is the main method for preparing flavors. Since the thermal reaction requires a large amount of proteolysis solution, the protein source has become one of the main factors restricting the efficiency of enzymatic hydrolysis. Finding excellent and cheap high-protein raw materials has become an obstacle to enzymatic hydrolysis....

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A23L1/221A23L27/10
Inventor田晶
OwnerTIANJIN CHUNFA BIO TECH GRP