Method for extracting huperzine A from huperzine serrate

Abstract

The invention discloses a method for extracting huperzine A from huperzine serrate. The method comprises the following steps of: preparing materials, extracting, leaching, performing column chromatography, crystallizing and the like, wherein according to the extracting step, a continuous countercurrent extraction method is adopted, so that the dissolution of active ingredients can be accelerated, the recovery rate of the huperzine A can be improved, and the extraction time can be shortened; according to the column chromatography, reversed phase chromatography is performed by cyano bonded silica gel resin, so that the recovery rate and purity can be improved by over 90 percent; and the reversed phase chromatography is performed by the cyano bonded silica gel resin, and the toxicity of an eluent is small.

Description

technical field [0001] The invention relates to a method for extracting huperzine A, in particular to a method for extracting huperzine A from a Melaleuca tower. Background technique [0002] With the rapid increase of the world's aging population, the number of patients with Alzheimer's disease (AD) is increasing, and it has become the third disease that threatens the life of the elderly after cardiovascular disease and tumor disease. Clinically, AD is mainly manifested as progressive cognitive decline, accompanied by behavioral disturbances and emotional abnormalities, until one cannot take care of oneself. Among the population over 65 years old, the prevalence rate of Westerners is about 5%, and the prevalence rate of people over 60 years old in my country is 3.4-3.9%. The pathological changes of AD are mainly diffuse atrophy of the cortex, widening of the sulci, enlargement of the ventricles, a large number of neurons, and senile plaques (SP), neurofibrillary knots (NFT...

AI Technical Summary

Problems solved by technology

[0006] At present, huperzine A is mainly extracted from the Chinese herbal medicine Melaleuca, and its main production steps are: raw material processing (selection, crushing)→dipping (soaking in acid water)→concentration→extraction→column chromatography→refining, etc., the yield bottom, the purity is limited, and the production scale is small, which cannot meet the market demand

[0007] Existing enterprises use organic solvent reflux extraction, ultrasonic extraction and microwave extraction, the recovery rate is high, but the purity of the product is low, which brings difficulties to the subsequent separation and purification; and the product purity of the acid water cold leaching and acid water percolation method is relatively high , but the extraction rate is low

In the refining method, existing enterprises adopt "high performance liquid chromatography" or "high-speed countercurrent chromatography" to separate Huperzine A. Although the content is high, the production volume is small, and most of them are limited to laboratory applications. If used in large-scale industrial Production requires not only expensive equipment but also very high quality requirements for technical operators; industrial production mostly uses resin chromatography for purification, and although the purity of the separated product is improved, the recovery rate is low

In addition, the distribution of Melaleuca pagoda resources is scattered, the resource renewal cycle is long, there are few natural resources available for exploitation, the price of medicinal materials has doubled, and the content of active ingredients in the whole plant is very low

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