Hepatitis A virus strain HAV-ZL2012, vaccine prepared by using same and application thereof

A technology of hepatitis A virus and virus strains, applied in the field of bioengineering, can solve the problems of long growth cycle and high production cost, and achieve the effects of weakened pathogenicity, shortened replication cycle, and weakened virus virulence
CN102757941AActive Publication Date: 2012-10-31内蒙古必威安泰生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
内蒙古必威安泰生物科技有限公司
Publication Date
2012-10-31

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Abstract

The invention discloses a hepatitis A virus strain HAV-ZL2012, a vaccine prepared by using same and application thereof. According to the invention, through the adoption of a reverse molecular biology technology and a novel subculturing method, the adaption and attenuation of a virus strain obtained through primary isolation are realized and rapidly facilitated; and growth, immunity and safety studies show that the virus strain HAV-ZL2012 can be used for preparing both live attenuated vaccines and inactivated vaccines. According to the invention, through the adoption of a rapid attenuation and adaption method of the hepatitis A virus, the growth period of the hepatitis A virus is reduced from 100 days to 14 days. The generation order of subculture adaption on a diploid cell is reduced from 40 generations to 10 generations; the generation order of subculture adaption on a subculture cell is reduced to 10-15 generations; the production period and cost are greatly reduced; and a great promoting effect on the development of a hepatitis A vaccine is obtained.
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Description

technical field

[0001] The present invention relates to a hepatitis A virus strain, a vaccine prepared from the virus strain and its application, in particular to a hepatitis A virus strain HAV-ZL2012, an attenuated live vaccine and an inactivated vaccine prepared therefrom. The invention also provides an attenuation adaptation method of the virus strain. Belongs to the field of bioengineering. Background technique

[0002] Viral hepatitis A, referred to as hepatitis A and hepatitis A, is caused by hepatitis A virus (Hapatitis Avirus, HAV). , Loss of appetite, hepatomegaly, and abnormal liver function are the main manifestations. In some cases, jaundice occurs. The main manifestation is acute hepatitis, which is common in asymptomatic infections. The disease can occur at any age, but mainly affects children and adolescents. The clinical symptoms of hepatitis A in adults are generally more severe than those in children. Winter and spring are often the peak period of hepat...

Claims

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