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Psychotria sp. extract, and preparation method and antineoplastic application thereof

A technology of Shandayan and its extracts, which is applied in the field of natural medicine and chemical medicine, can solve the problems that have not yet been reported on the anti-tumor research of Shandayan, and achieve the effects of good medicinal prospects, extensive resources, and easy large-scale production

Inactive Publication Date: 2012-11-07
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also a national intangible cultural heritage - one of the commonly used traditional Chinese medicines of Guangdong herbal tea, but there is no report on Shan Dayan's anti-tumor research

Method used

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  • Psychotria sp. extract, and preparation method and antineoplastic application thereof
  • Psychotria sp. extract, and preparation method and antineoplastic application thereof
  • Psychotria sp. extract, and preparation method and antineoplastic application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation of Shandayan extract

[0021] Dry 2.0kg of the stem of Psychotria sp. (produced in Conghua, Guangdong), crush it and pass it through a 20-mesh sieve, use 25L of 95% ethanol to filter and extract, filter the extract, concentrate under reduced pressure to extract (300g), Then add 2L of water, carry out macroporous resin enrichment, eluent methanol-water gradient elution (10 percentage points is a gradient), TLC tracking, 50~90% section collection, decompression concentration to remove solvent is Shandayan Extract (60g). As determined by HPLC, the mass content of total triterpene saponins was 88%. Wherein the content of 7 kinds of triterpene saponins is successively: 8.5% of 1, 22.8% of 2, 2.5% of 3, 6.0% of 4, 2.5% of 5, 5.0% of 6, 15.0% of 7; mass percentage.

Embodiment 2

[0022] Embodiment 2, the preparation of Shandayan extract

[0023] Dry 2.0kg of the stem of Psychotria sp. (produced in Conghua, Guangdong), crush it and pass it through a 20-mesh sieve, use 25L of methanol to filter and extract, filter the extract, concentrate under reduced pressure to extract (350g), and then add 2L of water, for macroporous resin enrichment, eluent methanol-water (10 percentage points as a gradient), TLC tracking, 65~90% section collection, decompression concentration to remove solvent is Shandayan extract (70g) . As determined by HPLC, the mass content of total triterpene saponins was 93%. Wherein the content of 7 kinds of triterpene saponins is successively: 9.0% of 1, 35.0% of 2, 2.5% of 3, 7.0% of 4, 2.5% of 5, 5.5% of 6, 14.0% of 7; mass percentage.

Embodiment 3

[0024] Embodiment 3, the preparation of Shandayan extract

[0025] Dry 2.0kg of the stem of Psychotria sp. (produced in Conghua, Guangdong), crush it and pass it through a 20-mesh sieve, heat and reflux with 60-80% methanol / water 25L to extract, filter the extract, concentrate under reduced pressure to extract (500g), then add 2L of water to enrich the macroporous resin, eluent methanol-water (10% is a gradient), TLC tracking, 55~90% section collection, decompression concentration to remove the solvent is Shanda Yan Extract (75g). As determined by HPLC, the mass content of total triterpene saponins was 95%. Wherein the content of 7 kinds of triterpene saponins is successively: 8.0% of 1, 37.0% of 2, 3.0% of 3, 7.0% of 4, 3.0% of 5, 4.5% of 6, 13.5% of 7; the percentage is mass percentage.

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Abstract

The invention discloses a Psychotria sp. extract, and a preparation method and antineoplastic application thereof. The preparation method of the Psychotria sp. extract comprises the following steps: carrying out heating reflux or percolation extraction on the Psychotria sp. by ethanol or methanol; carrying out decompression concentration to obtain an extract paste; enriching the extract paste by a macroporous resin; carrying out methanol-water washing to obtain the Psychotria sp. extract. The Psychotria sp. extract provided by the invention can be used in preparation of medicaments and health care products for treating tumor, has clear components in effect parts, low toxicity, strong pharmacological action, and good pharmaceutical prospect; besides, the preparation process is simple and easy for mass production.

Description

technical field [0001] The invention relates to the fields of natural medicines and chemical medicines, in particular to a preparation method of Shandayan extract and its main components and its application in antitumor. Background technique [0002] At present, there are about 4.5 million malignant tumor patients in my country, with more than 2 million new cases and about 1.7 million deaths every year. Malignant tumors have surpassed cardiovascular and cerebrovascular diseases and become the number one killer threatening human life [1] . Therefore, the research and development of anticancer drugs has always been a difficult and hot topic in the world of medicine. In my country, Chinese herbal medicine has been widely used in anti-tumor clinically, and has the characteristics of definite curative effect, low toxic and side effects, and not easy to produce drug resistance. [2-4] , has received more and more attention from medical workers. According to statistics, about 60...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P35/00C07J21/00A61K31/7048
Inventor 张翠仙张冬梅陈敏峰叶文才雷玲芳王志芳钟莹
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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