Bacteriacide composition for preventing main diseases of rice
A composition and rice sheath blight technology, applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of Jinggangmycin's reduced control effect
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Embodiment 1
[0019] Embodiment 1, the composition of different proportioning is to the virulence determination of rice sheath blight pathogenic bacteria
[0020] Two strains of rice sheath blight (Rhizoctonia solani) were collected from Jilin and Fujian respectively. Strain number: JHT158-3, FBS-3.
[0021] E medium: K 2 HPO 4 2g, KH 2 PO 4 2g, 14g agar powder, 10g glucose, 1000ml deionized water. Autoclave at 115°C for 15 minutes.
[0022] 1. The experimental steps are as follows:
[0023] 1) Mix Thiofuramide and Jinggangmycin with dimethyl sulfoxide to prepare 10 5 μg / ml stock solution, dilute thiofluxamide step by step to 1000μg / mL, 500μg / mL, 200μg / mL, 100μg / mL, 50μg / mL, 20μg / mL, dilute Jinggangmycin to 4×10 4 μg / mL, 1.6×10 4 μg / mL, 8×10 3 μg / mL, 4×10 3 μg / mL, 10 3 Concentration gradients of μg / mL and 500 μg / mL. Design 12 proportioning ratios of Thiofuramide and Jinggangmycin, respectively 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 2:3, 2:5, 3: 1, 3:2, 4:1, 5:1, each ratio has 6 conc...
Embodiment 2
[0049] Embodiment 2, field efficacy test of fungicide composition to rice sheath blight
[0050] Select the compound ratio with synergistic effect to construct the fungicide composition 16% thia.jing.mime suspension. According to the requirements of the "Pesticide Field Efficacy Test Guidelines" of the Pesticide Control Institute of the Ministry of Agriculture, field efficacy tests were carried out in Heilongjiang and Fujian provinces in my country. Design 3 different application concentrations of 16% thiium·jing·mimic acid suspension, 5 control agents and clear water control, a total of 9 treatments, each treatment was repeated 4 times, each plot was randomly arranged, and measured 15 days and 30 days after application The control effect of pesticides on major rice diseases and the safety of crops.
[0051] 3.1 Drugs to be tested
[0052] 3.1.1 Test drug
[0053] 16% thia.jing.mizu suspension concentrate: its detailed preparation method is as follows:
[0054] Using wet g...
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