Creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and preparation method thereof
A chemiluminescent immunoassay and creatine phosphokinase technology, which is applied in the field of immunoassay medicine, can solve the problems of narrow detection range and low sensitivity, and achieve the effect of simple operation, high sensitivity and good performance
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Embodiment 1
[0033] Example 1: Preparation of creatine phosphokinase isoenzyme (CK-MB) chemiluminescent immunoquantitative detection kit
[0034] Creatine phosphokinase isoenzyme (CK-MB) chemiluminescent immunoquantitative detection kit, characterized in that the kit includes: CK-MB antibody-coated plate, CK-MB antibody enzyme conjugate, CK-MB calibration products, CK-MB quality control products, 20 times concentrated lotion, luminescent solution A and B.
[0035] Creatine phosphokinase isoenzyme chemiluminescent immunoquantitative detection kit was prepared by the following method
[0036]1) Preparation of CK-MB antibody-coated plate: Dilute CK-MB antibody to 1-10ug / mL with 0.05mol / L pH9.6 carbonate buffer, add it to a white microwell plate, and coat at 37°C for 2 hours; discard the liquid in the wells, wash the plate with pH 7.4 PBS-T buffer, then add phosphate buffer containing 0.5% BSA to seal the microwell plate, and block for 2 hours at 37°C; discard the liquid in the wells, and sha...
Embodiment 2
[0058] Example 2: Preparation of CK-MB Chemiluminescent Immunoquantitative Detection Kit
[0059] The kit of the present invention was prepared in the same manner as in Example 1, except that the solid phase carrier was a 48-well white microwell plate.
Embodiment 3
[0060] Embodiment 3: the using method of kit of the present invention
[0061] 1) Equilibrate the test kit at room temperature (18-25°C) for 30 minutes.
[0062] 2) Prepare lotion: Dilute the concentrated lotion with distilled water at 1:20 (1mL lotion plus 19mL distilled water). If the concentrated lotion has crystals, place the concentrated lotion at room temperature or 37°C until the crystals dissolve before diluting.
[0063] 3) Preparation of luminous liquid: 5 minutes before use, take an appropriate amount of luminous liquid A and B and mix them in equal volumes.
[0064] 4) Take out an appropriate amount of coated slats. Set 1 well for blank control, 2 wells for calibrator, and 10 wells for quality control. Add 20 μL each of CK-MB calibrator, quality control substance, and sample to each well, and do not add calibrator, quality control substance, and sample to the blank control.
[0065] 5) Add 100 μL of CK-MB enzyme conjugate to each well, except for blank control ...
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