Device and method for flat membrane oxygen-supply self-circulation-type microbial culture

A microbial culture, self-circulating technology, applied in the field of microbial aerobic culture equipment, flat membrane oxygen supply self-circulating microbial culture device, can solve the problems of insufficient oxygen contact, achieve convenient combination, improve oxygen utilization, increase The effect of a large contact area
CN102816689BInactive Publication Date: 2013-10-16BEIJING INSTITUTE OF TECHNOLOGYGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING INSTITUTE OF TECHNOLOGYGY
Publication Date
2013-10-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to microbial aerobic culture equipment and especially relates to a device and a method for flat membrane oxygen-supply self-circulation-type microbial culture and belongs to the technical field of environmental protection. The device comprises an air feed pump, an inlet valve, a gas flow meter, a heater, a liquid seal bottle, a gas storage tank, an air intake, a hydrophobic microporous flat membrane, a membrane support porous plate, a self-circulation guide cylinder, a feeding and sampling opening, an inoculation opening, a gas outlet, an upper cover and an outer wall. Air is dispersed into air bubbles by the hydrophobic microporous flat membrane, passes through the membrane support porous plate and then enters into a cavity of a culture container. Based on relative positions of ventilative small apertures in the membrane support porous plate and the self-circulation guide cylinder, a lifting liquid medium forms external circulation relatively to the self-circulation guide cylinder and also forms inner circulation. Through culture liquid circulation, microbes in the liquid medium suspend so that the microbes fully contact with air. The device and the method solve the problem that a traditional membrane bioreactor needs an additional air filter and a disturbance or stirring device.
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Description

technical field

[0001] The invention relates to microorganism aerobic cultivation equipment, in particular to a flat membrane oxygen supply self-circulation microorganism cultivation device and method, belonging to the field of microorganism cultivation and membrane technology. Background technique

[0002] At present, for microbial aerobic culture and cell fermentation to produce enzymes, microorganisms are usually inoculated into a conical flask containing a microbial growth medium, and then the mouth of the conical flask is wrapped with multi-layer gauze and placed in a constant temperature oscillator for cultivation. Oxygen culture often requires oxygen for respiratory metabolism, and the constant temperature oscillator has ventilation equipment. During the shaking process of the triangular flask, the circulating air is immersed in the triangular flask through gauze and dissolved in the medium, so that the microorganisms can perform respiratory metabolism.

[0003] The r...

Claims

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