Device and method for flat membrane oxygen-supply self-circulation-type microbial culture

A microbial culture, self-circulating technology, applied in the field of microbial aerobic culture equipment, flat membrane oxygen supply self-circulating microbial culture device, can solve the problems of insufficient oxygen contact, achieve convenient combination, improve oxygen utilization, increase The effect of a large contact area

Inactive Publication Date: 2013-10-16
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of insufficient oxygen contact in the existing microbial aerobic culture and cell fermentation enzyme production process, and the oxygen passing process must be provided with an air filter device and a disturbance or stirring device, etc., to provide a flat membrane oxygen supply Self-circulating microbial culture device and method

Method used

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  • Device and method for flat membrane oxygen-supply self-circulation-type microbial culture
  • Device and method for flat membrane oxygen-supply self-circulation-type microbial culture
  • Device and method for flat membrane oxygen-supply self-circulation-type microbial culture

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Embodiment 1

[0032] A kind of flat membrane oxygen self-circulation type microbial cultivation device and method, comprising: air supply pump 1, air intake valve 2, air flow meter 3, incubator 4, heater 5, liquid-sealed bottle 6; figure 1 As shown; after the air supply pump 1, the air intake valve 2, and the air flow meter 3 are connected in sequence through pipelines, they are then connected to the air inlet 402 of the incubator 4, and the incubator 4 is placed in the heater 5, and the incubator 4 The gas outlet 408 is placed below the liquid level of the liquid-sealed bottle 6 with a vent tube to carry out liquid sealing to prevent microorganisms from contaminating bacteria.

[0033]Wherein, the incubator 4 includes: gas storage tank 401, air inlet 402, hydrophobic microporous flat membrane 403, membrane support orifice plate 404, self-circulation guide tube 405, feeding and sampling port 406, inoculation port 407, gas outlet 408, upper cover 409, outer wall 4010, first washer 4011, seco...

Embodiment 2

[0043] A kind of flat membrane oxygen self-circulation type microbial cultivation device and method, comprising: air supply pump 1, air intake valve 2, air flow meter 3, incubator 4, heater 5, liquid-sealed bottle 6; figure 1 As shown; after the air supply pump 1, the air intake valve 2, and the air flow meter 3 are connected in sequence through pipelines, they are then connected to the air inlet 402 of the incubator 4, and the incubator 4 is placed in the heater 5, and the incubator 4 The gas outlet 408 is placed below the liquid level of the liquid-sealed bottle 6 with a vent tube to carry out liquid sealing to prevent microorganisms from contaminating bacteria.

[0044] Wherein, the incubator 4 includes: gas storage tank 401, air inlet 402, hydrophobic microporous flat membrane 403, membrane support orifice plate 404, self-circulation guide tube 405, feeding and sampling port 406, inoculation port 407, gas outlet 408, upper cover 409, outer wall 4010, first washer 4011, sec...

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PUM

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Abstract

The invention relates to microbial aerobic culture equipment and especially relates to a device and a method for flat membrane oxygen-supply self-circulation-type microbial culture and belongs to the technical field of environmental protection. The device comprises an air feed pump, an inlet valve, a gas flow meter, a heater, a liquid seal bottle, a gas storage tank, an air intake, a hydrophobic microporous flat membrane, a membrane support porous plate, a self-circulation guide cylinder, a feeding and sampling opening, an inoculation opening, a gas outlet, an upper cover and an outer wall. Air is dispersed into air bubbles by the hydrophobic microporous flat membrane, passes through the membrane support porous plate and then enters into a cavity of a culture container. Based on relative positions of ventilative small apertures in the membrane support porous plate and the self-circulation guide cylinder, a lifting liquid medium forms external circulation relatively to the self-circulation guide cylinder and also forms inner circulation. Through culture liquid circulation, microbes in the liquid medium suspend so that the microbes fully contact with air. The device and the method solve the problem that a traditional membrane bioreactor needs an additional air filter and a disturbance or stirring device.

Description

technical field [0001] The invention relates to microorganism aerobic cultivation equipment, in particular to a flat membrane oxygen supply self-circulation microorganism cultivation device and method, belonging to the field of microorganism cultivation and membrane technology. Background technique [0002] At present, for microbial aerobic culture and cell fermentation to produce enzymes, microorganisms are usually inoculated into a conical flask containing a microbial growth medium, and then the mouth of the conical flask is wrapped with multi-layer gauze and placed in a constant temperature oscillator for cultivation. Oxygen culture often requires oxygen for respiratory metabolism, and the constant temperature oscillator has ventilation equipment. During the shaking process of the triangular flask, the circulating air is immersed in the triangular flask through gauze and dissolved in the medium, so that the microorganisms can perform respiratory metabolism. [0003] The r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/04C12M1/00C12N1/00
CPCC12M41/00C12M29/16C12M41/12C12M23/38C12M27/24
Inventor 赵之平石教育王可达李梅生陈康成
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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