Composition for the determination of coagulation characteristics of a test liquid

Abstract

The present invention is directed to a diagnostic composition for use in the viscoelastic analysis of a test liquid, and to a container (1) comprising same. The composition comprises at least an activator of coagulation, and at least one further constituent selected from CaCl2 and from one or more inhibitors and / or coagulation components, wherein the composition is present in essentially dry form of all constituents and in an amount sufficient for performing one single viscoelastic analysis of a specified blood or plasma sample and wherein the constituents are not present in a substance mixture, but in a spatially separated form. The present invention is further directed to a method of performing a viscoelastic analysis on a test liquid, and to the use of the diagnostic composition in such a method.

Description

technical field [0001] The present invention relates to diagnostic compositions for viscoelastic analysis of test fluids and to containers containing the diagnostic compositions. The invention further relates to methods for viscoelastic analysis of test liquids, and to the use of diagnostic compositions in such methods. Background technique [0002] Coagulation of blood is a complex process in which blood forms a solid clot. It is an important part of hemostasis (stopping blood loss from damaged blood vessels), whereby the walls of damaged blood vessels are protected by clots to stop bleeding and aid in the repair of damaged blood vessels. Disorders in coagulation can lead to increased bleeding and / or thrombosis and embolism. [0003] In normal individuals, clotting begins within 20 seconds after injury to blood vessels that damages endothelial cells. Platelets immediately form a hemostatic plug at the site of injury. This process is called primary hemostasis. Assisted ...

AI Technical Summary

Problems solved by technology

Some components can negatively affect each other's stability when staying together in higher concentrations in the liquid phase; for example, in the liquid phase, over time, CaCl 2 Can disrupt the stability of tissue factor reagents

[0029] In addition, if these combined reagents are provided in an amount sufficient for exactly one test, another problem arises: in this case, a very small part of the liquid reagent may adhere to part of the reagent container or cup, thus when performing Not sufficiently mixed with the test liquid for analysis

[0031] However, the disadvantages of this solution include the need for a very precise pipetting process, since the separate reagent chambers are very small, and the problem that, before freeze-drying, the reagent drops still possible to mix

However, the following problem arises here: all the components required for the analysis are in direct contact with each other

Thus, by using substances in this form, problems with long-term stability or incompatibility can arise

Images