Formula of Chaetoceros muelleri medium and white plastic barrel aerated culture method

A technology of Chaetoceros mouvii and culture medium formula, which is applied in the field of marine microalgae cultivation, and can solve the problems of high cost, easy pollution, easy scratching, etc.

Inactive Publication Date: 2013-01-02
LINYI UNIVERSITY
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AI Technical Summary

Problems solved by technology

In terms of production, glass bottles are generally used for primary conservation, small ponds or small film bags or glass tanks for secondary expansion, and...

Method used

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Experimental program
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Embodiment Construction

[0015] Culture medium preparation method

[0016] 1) Ferric citrate is difficult to dissolve, you can add a small amount of tap water and heat it to 80-90°C on the stove, and keep stirring until it completely melts;

[0017] 2) 920 plant growth stimulant (ie gibberellin) is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved with alcohol first (generally, 50 ml of alcohol or 60-degree liquor per gram is dissolved for more than 1 hour), and then Dilute with water to desired concentration.

[0018] 3) During the process of adding nutrient salts, the water needs to be continuously stirred, and various nutrient salts are added in the order provided in the formula to avoid chemical reactions;

[0019]4) Various nutrient salts can be made into mother liquor with higher concentration first, and then added in sequence.

[0020] 5) After adding all the nutrient salts, inflate and stir for 15 minutes before inoculating.

[0021] 6) This medi...

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Abstract

The invention belongs to the field of marine microalgae cultivation, and relates to a formula of a Chaetoceros muelleri medium and a white plastic barrel aerated culture method. Chaetoceros muelleri is a small marine diatom, rich in polyunsaturated fatty acid and protein, and is a high-quality bait for raising sea treasures such as prawn and abalone. At present, an f / 2 culture medium formula used to produce Chaetoceros muelleri has problems of imbalanced and incomprehensive nutrients, slow growth, long multiplication time and low yield. ccording to experiments, nitrate is substituted by urea, which is used as a nitrogen source and supplemented by NaHCO3 and growth hormone, VB12 and VB1, so that the medium gains more comprehensive and balanced nutrients, and yield of Chaetoceros muelleri is increased by 87%. In addition, cement pool and glass cylinder barrel used for culture in current production have many disadvantages: easy pollution, difficult washing, inconvenient operation and easy cutting. Study in the invention shows that a white plastic barrel aerated culture method for culturing Chaetoceros muelleri has advantages of flexible operation, no pollution, easy washing, no cutting, low cost, high yield and large-scale promotion.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a culture medium formula of Chaetoceros mouroii and a method for inflating a white plastic barrel for cultivation. Background technique [0002] Chaetoceros muelleri (Chaetoceros muelleri) belongs to the diatom door, central class, box-shaped algae, Chaetoceros family, Chaetoceros genus, is a small marine planktonic diatom, the cells are small, the wall is thin, and most of them live alone , can grow and reproduce at an ambient temperature of 10-39°C, and the optimum temperature is 25-30°C. It is a coastal brackish water species. Compared with other commonly used unicellular algae, such as Isochrysis galbana Parke, Dicrszhan jiangensis and Platymonas, Chaetoceros mouchei contains a large amount of nutrients such as fatty acids and proteins, and is very suitable for reproduction. Fast and high temperature resistant. The content of polyunsaturated fatty ac...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner LINYI UNIVERSITY
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