Formula of Chaetoceros muelleri medium and white plastic barrel aerated culture method
A technology of Chaetoceros mouvii and culture medium formula, which is applied in the field of marine microalgae cultivation, and can solve the problems of high cost, easy pollution, easy scratching, etc.
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[0015] Culture medium preparation method
[0016] 1) Ferric citrate is difficult to dissolve, you can add a small amount of tap water and heat it to 80-90°C on the stove, and keep stirring until it completely melts;
[0017] 2) 920 plant growth stimulant (ie gibberellin) is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved with alcohol first (generally, 50 ml of alcohol or 60-degree liquor per gram is dissolved for more than 1 hour), and then Dilute with water to desired concentration.
[0018] 3) During the process of adding nutrient salts, the water needs to be continuously stirred, and various nutrient salts are added in the order provided in the formula to avoid chemical reactions;
[0019]4) Various nutrient salts can be made into mother liquor with higher concentration first, and then added in sequence.
[0020] 5) After adding all the nutrient salts, inflate and stir for 15 minutes before inoculating.
[0021] 6) This medi...
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