Method for preparing super cytokine induced killer (CIK) cells

A cell-killing, super technology, applied in the medical field, can solve problems such as serious toxic and side effects, and achieve the effects of reducing side effects, avoiding toxic side effects, and avoiding immune escape

Inactive Publication Date: 2013-01-30
扬州维克斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Systemic administration not only activates T cells in tumor tissue, but also greatly activates T cells in the blood circulation of the whole body and in various organs, and its toxic and side effects are also very serious

Method used

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  • Method for preparing super cytokine induced killer (CIK) cells
  • Method for preparing super cytokine induced killer (CIK) cells
  • Method for preparing super cytokine induced killer (CIK) cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed.

[0024] 1. Preparation of CIK cells

[0025] 1. The preparation of super CIK cells of the present invention, such as figure 1 Shown:

[0026] (1) Collection and preparation of peripheral blood mononuclear cells (PBMC)

[0027] Use a blood cell separator to collect anticoagulant blood from tumor patients under sterile conditions, add Ficoll-Paque Plus and blood with a specific gravity of 1.077 to a centrifuge tube at a ratio of 1:2, centrifuge at 1500rpm / 30min, and take interface layer cells, PBS Wash twice, suspend in serum-free GT-T561 medium, adjust cell concentration to 1×10 6 cells / mL.

[0028] (2) Preparation of super CIK killer cells

[0029] In the PBMC cells prepared on the same day, add , at 37°C, 5% CO 2 cultured in an incubator.

[0030] After 24 hours, add C...

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Abstract

The invention discloses a method for preparing super cytokine induced killer (CIK) cells, and belongs to the technical field of medical treatment. By the method, after in vitro amplification tumor killer cells reach the required quantity, cytotoxic T lymphocyte antigen (CTLA4) antibodies are in vitro loaded to cover all CTLA4 molecules on the surfaces of the cells; and all surplus CTLA4 antibodies are washed off after the loading is finished. After the prepared high-activity tumor killer cells are returned by veins, the CTLA4 antibodies achieve the curative effect of the antibody drug, the serious toxic or side effect caused by injecting a large dosage of CTLA4 antibodies from the veins can be avoided, the tumor cells can be continuously killed, and the aims of inhibiting the tumor tissues for long time and even clearing the tumor tissues are finally fulfilled.

Description

technical field [0001] The invention belongs to the field of medical technology, in particular to a method for preparing killer cells used for treatment in tumor biotechnology. Background technique [0002] Tumor biotherapy is the fourth treatment after surgery, radiotherapy and chemotherapy. Cytokine-induced killer cells (CIK) therapy is currently the most effective biological therapy for tumors. CIK cells are broad-spectrum anti-tumor immune cells with strong proliferative ability in vitro, high tumor-killing activity and sensitivity to multidrug-resistant tumor cells. [0003] At present, the CIK cells prepared at home and abroad for tumor immunotherapy are heterogeneous cell populations mainly expanded in vitro, mainly CD3+CD56+, CD3+CD8+. The cell population is stimulated by various cytokines such as IL2, IFN-γ and monoclonal antibody (CD3McAb), cultured and expanded in vitro from mononuclear cells isolated from peripheral blood, bone marrow or umbilical cord blood. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
Inventor 胡伟明
Owner 扬州维克斯生物科技有限公司
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