Box-type cultivation method of tussah pupa cordyceps militaris

Abstract

The invention relates to a box-type cultivation method of tussah pupa cordyceps militaris. The method comprises the steps of tussah pupa cleaning, sterilization, inoculation and cordyceps militaris cultivation. The strain is cultivated via a PDA liquid medium; and the strain is cultivated in a table concentrator at a temperature of about 23 DEG C till the culture solution is viscous and fungus balls appear after the medium is sterilized and inoculated at a high temperature. The cultivated liquid strain is filtered to an aseptic bottle; the fungus balls are washed with sterile water for 2-3 times, and the volume of the sterile water 0.5-1 time that of the bacteria liquid; and the whole process is finished through aseptic operations. During the inoculation, the bacteria liquid is absorbed and injected at a second section from the tail of the tussah pupa via an injector; after the inoculation, a single layer of the tussah pupa is put in a sterilized culture room; after the inoculated part scabs, the tussah pupas are transferred to a sterilized plastic box for cultivation; after the tail of the tussah pupa is rigidified and hardened and mycelia appear at the section, the tussah pupa is put in a box with air vents; the temperature and the humidity are kept till cordyceps militaris comes up; then the cordyceps militaris is collected and dried. The method has the advantages that the temperature and the humidity are easy to control and adjust, illumination is sufficient, collection is easy, cultivation time of the cordyceps militaris is short, the operation is convenient, growth of the cordyceps militaris is tidy, high success rate of 95% or higher can be achieved and commodity of the cordyceps militaris is good.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a box-type cultivation method of tussah silkworm and cordyceps militaris. Background technique [0002] Cordyceps militaris is also known as Cordyceps militaris, Cordyceps militaris, Cordyceps militaris, etc. Its active ingredients are similar to those of Cordyceps militaris. [0003] Wild Cordyceps militaris resources are scarce in nature, and its output is greatly affected by the natural environment and climate, which cannot meet people's needs at all. Most of the Cordyceps militaris products on the market are artificially cultivated. The artificial cultivation methods are: (1) Collect wild Cordyceps militaris to isolate and purify the strains, then inoculate Cordyceps militaris on solid medium, and cultivate them at 20-25°C under certain humidity and light conditions. After 35-45 days, fruiting bodies can be harvested. receive. Jiang Minglan et al. used w...

AI Technical Summary

Problems solved by technology

[0004] At present, the use of rice fermentation is also an important way to obtain the fruiting body of Cordyceps, but due to the lack of nutrients such as protein in the insect body in rice, the content of active ingredients such as cordycepin in the obtained fruiting body is low, and the quality is difficult to guarantee. Lawbreakers replace organic nitrogen with inorganic nitrogen sources, so that the content of the main active ingredient cordycepin in the obtained fruit bodies is very low, or even no, which interferes with the market of Cordyceps militaris