RT-HDA (Reverse Transcription-Helicase-Dependent Isothermal Amplification) kit and primer for detecting foot-and-mouth disease virus

Abstract

The invention discloses an RT-HDA (Reverse Transcription-Helicase-Dependent Isothermal Amplification) kit and primers for detecting a foot-and-mouth disease virus. The s for detecting a foot-and-mouth disease virus are oligonucleotide sequences shown in the sequence list SEQ ID No:1 and the sequence table SEQ ID No:2. In the invention, the novel isothermal amplification technology HDA is applied to the detection of the foot-and-mouth disease virus for the first time. Compared with the traditional FDMV detection method, the HDA detection method is quicker, more convenient, safer and more reliable. The traditional virus isolation and serological method has long time consumption and biosafety risk. The HDA method imitates the natural replication mechanism in the body and has high safety and quick response, and only 2 h are needed at most to complete detection. Compared with PCR (Polymerase Chain Reaction), the HDA method does not need any PCR instrument, the equipment requirement is simple, only one water bath kettle is needed, and the HDA method is easier to master and operate speaking from the technology level, is easier to be popularized and applied to regions with insufficient conditions and is more conducive to the diagnosis and monitoring of the foot-and-mouth disease virus.

Description

technical field

[0001] The invention relates to an RT-HDA kit and primers for detecting foot-and-mouth disease virus, belonging to the field of inspection and quarantine. Background technique

[0002] Foot-and-mouth disease (FMD) is an acute febrile highly contagious infectious disease caused by Foot-and-mouth disease virus (FMDV). It mainly infects artiodactyls. The mouth, tongue, and Blisters occur on lips, hooves, breasts and other parts, and ulceration forms rotten spots. Humans and non-cloven-hoofed animals can also be infected with the disease, but the symptoms are milder. Because pigs, cattle, sheep and other major domestic animals can be infected with the disease, and can form a large-scale global epidemic, the World Organization for Animal Health (OIE) and the Food and Agriculture Organization of the United Nations (FAO) have listed the disease as a category A zoonotic disease. In my country, foot-and-mouth disease is listed as a class of animal infectious disease...

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