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Tools and method for nanopores unzipping-dependent nucleic acid sequencing

A nucleic acid sequencing and nanopore technology, which is applied in biochemical equipment and methods, biological testing, microbial measurement/inspection, etc., and can solve problems such as nanopore difficulties

Inactive Publication Date: 2013-02-06
TRUSTEES OF BOSTON UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the fabrication of nanopores can produce large numbers of synthetic nanopores, it is difficult to fabricate nanopores with very small pores in a uniform and constant quality.

Method used

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  • Tools and method for nanopores unzipping-dependent nucleic acid sequencing
  • Tools and method for nanopores unzipping-dependent nucleic acid sequencing
  • Tools and method for nanopores unzipping-dependent nucleic acid sequencing

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Experimental program
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Embodiment

[0221] Optical recognition of individual nucleobases in single-molecule DNA sequencing using nanopore arrays

[0222] introduce

[0223] High-throughput DNA sequencing technology is profoundly impacting comparative genomics, biomedical research, and personalized medicine 1 . Specifically, single-molecule DNA sequencing techniques minimize the amount of DNA material required and are thus considered outstanding candidates for providing low-cost and high-throughput sequencing methods targeting a broad range of DNA read lengths 1-4 . Solid-state nanopores are a class of single-molecule probe technologies with broad applications, including characterizing DNA structure and DNA-drug or DNA-protein interactions 5-12 . Unlike other single-molecule techniques, detection using nanopores does not require immobilization of macromolecules to a surface, thus simplifying sample preparation. Additionally, solid-state nanopores can be fabricated in a high-density format, which would allow ...

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Abstract

Provided herein is a library that comprises a plurality of molecular beacons (MBs), each MB having a detectable label, a detectable label blocker and a modifier group. The library is used in conjunction with nanopore unzipping-dependent sequencing of nucleic acids.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit under 35 U.S.C. §119(e) of U.S. Provisional Application No. 61 / 318,872, filed March 30, 2010, the contents of which are hereby incorporated by reference in their entirety. [0003] Government funding [0004] This invention was made with US Government support under Contract No. RO1-HG004128 awarded by the National Institutes of Health. The US Government has certain rights in this invention. Background of the invention [0005] Nanopore sequencing is a promising technology developed as an inexpensive and rapid alternative to conventional Sanger sequencing methods. Nanopore sequencing methods may offer several advantages over conventional Sanger sequencing methods; they allow single-molecule analysis, are not enzyme-dependent (eg, no polymerase is required for chain extension) and require significantly fewer reagents. [0006] A number of nanopore-based DNA sequencing methods have bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/48G01N33/58
CPCC12Q1/6874C12Q1/6869C12Q1/682C12Q2565/1015C12Q2525/151C12Q2565/631
Inventor 阿米特·梅勒阿龙·辛格
Owner TRUSTEES OF BOSTON UNIV