Molecular vector for single molecule detection
A single-molecule detection and molecular technology, which is used in material excitation analysis, Raman scattering, etc., can solve the problems of low resolution, limited application range, insufficient Raman scattering signal, etc., achieve radiation signal enhancement, improve resolution and The effect of accuracy
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[0041] The preparation method of the molecular carrier 10 mainly includes: step (S111), providing a mother board 1001; step (S112), forming a three-dimensional nanostructure 102 on the surface of the mother board 1001, forming the substrate 100; step (S113) , forming a metal layer 101 on the surface of the substrate 100 to form the molecular carrier 10 .
[0042] In step ( S111 ), the motherboard 1001 may be an insulating material or a semiconductor material. The material of the motherboard 1001 in this embodiment is silicon dioxide. The thickness of the motherboard 1001 is 200 microns to 300 microns. The size, thickness and shape of the motherboard 1001 are not limited, and can be selected according to actual needs.
[0043] Further, a surface of the motherboard 1001 may be subjected to hydrophilic treatment.
[0044] Firstly, the surface of the motherboard 1001 is cleaned by using a standard cleaning process in a clean room. Then, at a temperature of 30°C~100°C, the volu...
no. 1 example
[0089] Compared with the first embodiment, the molecular carrier 40 provided by the second embodiment of the present invention, since the three-dimensional nanostructure 402 is a raised double-layer cylindrical structure, forms two different distances between adjacent double-layer cylindrical structures. Gap, that is, a gap is formed between adjacent first cylinders 404 , and another gap is formed between adjacent second cylinders 406 . Therefore, when the molecular carrier is used for single-molecule detection, under the excitation of the laser light emitted by the detector, the metal layer 401 in the gap between the adjacent first cylinders 404 generates surface plasmon resonance, while the second The metal layer 401 in the gap between the cylinders 406 produces plasmon resonance, which enhances the Raman scattering on the surface of the metal layer, so that the SERS enhancement factor can be further improved, the Raman spectrum can be enhanced, and the resolution of the sing...
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