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Novel method for screening mutagenic polyploids of jujube test-tube seedlings

A test-tube seedling and polyploid technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long purification and cultivation time, large number of chimeras, and few tetraploid mutants, etc.

Inactive Publication Date: 2014-05-28
YANAN UNIV
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a new method for mutagenic polyploid screening of jujube test-tube seedlings, aiming to solve the problem that 1. the mutagenesis rate refers to the number of chromosome number changes, and the follow-up reports are less; Many, long time for purification and culture; ③ The problem of few tetraploid mutants obtained by purification

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  • Novel method for screening mutagenic polyploids of jujube test-tube seedlings

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Embodiment Construction

[0021] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] step one:

[0023] The jujube jujube was cut into branches with a length of about 20 cm and brought back to the laboratory. First rinse under tap water for 10-15 minutes, brush the branches lightly with a soft brush while rinsing, soak the branches in 10% laundry solution for 30-60 minutes, then use 30ml of 84 disinfectant and add distilled water to 500ml to soak for 15 minutes. After cleaning with bacterial water, transfer to ultra-clean workbench, sterilize with 75% ethanol + 1 drop of Tween 20 for 10-15 seconds, then transfer to 0.1% mercuric chloride + 1 drop of Tween 20 for sterilizati...

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Abstract

The invention discloses a novel method for screening mutagenic polyploids of jujube test-tube seedlings. The novel method comprises the following steps of: (1) basic procedures of jujube test-tube seedling mutation; (2) carrying out first screening on the mutated plants according to the morphological characteristics; (3) carrying out differentiation culture and carrying out second screening on the differentiated multiple shoots; (4) carrying out chromosome analysis on the screened target multiple shoots; and (5) culturing the determined polyploids. The method has the beneficial effects that the mutagenic polyploids of jujube test-tube seedlings are screened by adopting the way of proceeding from the exterior to the interior in combination with continuous differentiation culture, so the selection rate of the tetraploids of the jujube test-tube seedlings can be greatly increased and the time can be shortened; Continuous three-time tests carried out on the jujubes by applying the method help discover that more than 35.0% of tetraploids can be bred within 110-150 days.

Description

technical field [0001] The invention relates to a new method for polyploid screening of jujube test-tube seedlings induced by mutagenesis, and belongs to the field of plant biotechnology and plant genetic breeding or the field of plant test-tube mutagenesis breeding. Background technique [0002] my country is the birthplace of jujube trees, and the valley of the Yellow River is one of the birthplaces and eugenic areas of jujube trees. Jujube tree planting resources are abundant in northern Shaanxi. Jujube is a plant of the genus Zizyphus jujube Mill Muzao in the family Rhamnaceae. It is recorded in "Chinese Fruit Tree Records Jujube Roll" that its fruit is medium-large, cylindrical, slightly flattened on the side, with an average fruit weight of 14.1 g, thick flesh, green White, hard texture, slightly thick, less juice, sweet and slightly sour. Contains 28%~33% soluble solids, 21.7% total sugar, 0.79% acid, 461.7 mg / l00 g vitamin C, 68% water content, 96.8% edible rate, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 齐向英张向前陈宗礼陈国梁薛皓高朋涛
Owner YANAN UNIV
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