Fixing device for suckling mouse brain extraction

Abstract

The invention discloses a fixing device for suckling mouse brain extraction. The fixing device comprises a black base flat dish, wherein four brackets which are uniformly distributed are arranged at the center of the base flat dish, an operating flat dish is supported by the four brackets together, a rough soft plastic thick film covers the bottom of the operating flat dish, two upright columns made from soft plastics are arranged on a plane of the bottom of the operating flat dish, a clamping groove exists in the middle of the two upright columns, and holes communicated with the base flat dish are formed in the clamping groove. The fixing device for the suckling mouse brain extraction has the important significance that the quality for suckling mouse brain tissue extraction can be effectively improved, the time for extraction is shortened, the pollution is reduced, the depletion rate of fine forceps is reduced, the workload for researchers is reduced, the fixing device is reusable, the cost for experimental consumables is reduced, and the like. Shown by document retrieval carried out by the applicant, similar devices of the same type do not exist yet.

Description

technical field [0001] The invention belongs to a basic biology experiment device, and relates to a device for extracting brain tissue from the culture of animal brain cells in basic medicine, and in particular to a brain-taking and fixing device for suckling mice. Background technique [0002] Animal cell culture, especially the brain cell (neuron, glial cell and vascular endothelial cell) culture of brain tissue such as cortex and hippocampus, is widely used in the research of nervous system and developmental biology. Using successfully cultured cells to make models of various diseases of the nervous system (brain injury, epilepsy and degenerative diseases, etc.), has become one of the important tools for studying the pathophysiological mechanism of the above diseases and guiding clinical treatment. [0003] It is a complex and time-consuming process from taking cortex, hippocampus and other tissues in suckling mice to the final successful cell culture and modeling. Every ...

AI Technical Summary

Problems solved by technology

[0005] 1) Glass dishes or plastic dishes are generally transparent, and the color contrast is extremely poor. Generally, when taking brains, the bottom of the dish is lined with black paper or a microscope with a black base is used, which is extremely inconvenient to use, and it is not economical to change the paper repeatedly;

[0006] 2) The bottom of the glass dish or plastic dish is smooth, and the suckling mouse is not well fixed, especially unable to stand upright, which greatly prolongs the operation time, affects the activity of brain tissue, and is low in cost performance;

[0007] 3) The bottom of the glass dish or plastic dish is smooth, and the suckling mouse is not fixed well. Generally, fine tweezers are used to fix it to keep it in a certain position

First of all, suckling mice are relatively fragile, and fine tweezers are easy to destroy more tissues to play a fixed role, and the effect is not good; secondly, fine tweezers are very expensive, and their tips are very fine, while the bottom of the plate is hard, and it is very easy to damage the tips of the tweezers during the fixation process. The loss rate is extremely high, resulting in great waste;

[0008] 4) In order to prevent the blood from affecting the purity of the extracted brain cells, dry glass dishes or plastic plates are generally selected. However, when there are many suckling rats, the blood on their bodies will accumulate more and more, resulting in a greater impact on the brain tissue of suckling rats extracted later. , decreased activity, poor effect

[0009] Through the literature search done by the applicant, so far, there is no report of similar similar devices

Images