Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight

A technology of microorganisms and wilt, which is applied to the treatment of biological organic parts, organic fertilizers, and the preparation of organic fertilizers. Stress resistance, no environmental pollution effect

Inactive Publication Date: 2013-04-03
KUNMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Panax notoginseng is a perennial herbaceous plant. Due to the long growth cycle of Panax notoginseng and harsh growth conditions (requiring warm winter and cool summer, no severe cold and heat, a specific environment with half sun and half shade, and humidity), the planting area is limited and only distributed in Counties in Wenshan City, Yunnan Province, and several places at the border between Guangxi and Wenshan[4], therefore, like ginseng and American ginseng in the same genus, there are serious continuous cropping obstacles[5], that is, the land where a crop of Panax notoginseng has been planted should be separated by at least eight It takes years or even decades to replant Panax notoginseng. If the crop rotation period is shortened, the aboveground parts of Panax notoginseng will show poor growth, many diseases, low yield, or even destroy the garden[6]
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Method used

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  • Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight
  • Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight
  • Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Preparation of microbial organic phosphate fertilizer for preventing and treating Panax notoginseng wilt

[0036] (1) Preparation of biocontrol bacteria seed solution

[0037] a. Transplant the KMXY1 strain with the preservation number CGMCC NO. 6598 into a test tube containing LB medium for primary activation, and culture at 29°C for 48 hours. The LB medium formula is: tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 5g / L, pH7.0, agar 17g / L;

[0038] b. Take a ring of activated bacterial lawn and transfer it into 600ml of 1000ml Erlenmeyer flask made of tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 5g / L, In the LB culture medium composed of pH 7.0, cultivate it at 110 rpm and 29 °C for 72 hours to obtain the fermentation broth;

[0039] c. Inoculate the fermentation broth in the same LB broth as in step b in the seed tank according to the inoculum ratio of 1:200, and ferment at 29°C or 30...

Embodiment 2

[0052] 1. Preparation of microbial organic phosphate fertilizer for preventing and treating Panax notoginseng wilt

[0053] The preparation steps of the microbial organic phosphate fertilizer for preventing and treating Panax notoginseng wilt are the same as in Example 1.

[0054] 2. Application of microbial organic phosphate fertilizer to prevent and control Panax notoginseng wilt

[0055] From 2009 to 2011, relevant experiments were carried out in Wenshan Prefecture, Yunnan Province. The physical and chemical properties of the tested farmland soil were basically the same, and the fertility was medium.

[0056] Random design was adopted, and three treatments were set up: continuous cropping soil, continuous cropping soil with this fertilizer application, and new land (not yet planted with Panax notoginseng), each treatment was repeated 3 times, and the area of ​​the plot was 200m 2 . In November 2009, apply this fertilizer embodiment as a base fertilizer to the soil at one ...

Embodiment 3

[0060] 1. Preparation of microbial organic phosphate fertilizer for preventing and treating Panax notoginseng wilt

[0061] The preparation steps of the microbial organic phosphate fertilizer for preventing and treating Panax notoginseng wilt are the same as in Example 1.

[0062] 2. Application of microbial organic phosphate fertilizer to prevent and control Panax notoginseng wilt

[0063] From 2009 to 2011, relevant experiments were carried out in Wenshan Prefecture, Yunnan Province. The physical and chemical properties of the tested farmland soil were basically the same, and the fertility was medium. Using random design, set up continuous cropping soil, continuous cropping soil with this fertilizer application and new land (not yet planted Panax notoginseng)

[0064] 3 treatments, each treatment repeated 3 times, the area of ​​the plot is 200m 2 . In November 2009, apply this fertilizer embodiment as the base fertilizer to the soil at one time, and the dosage is 25000kg...

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Abstract

The invention relates to a microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight, belonging to the technical field of microbial organic phosphate fertilizers. The microbial organic phosphate fertilizer is prepared by the steps of preparing biocontrol bacterium seed solution through liquid fermentation cultivation by using bacillus amyloliquefaciens; mixing and fermenting crushed water hyacinth residue, grass peat, humic acid and powdered rock phosphate with content of 20-24% and fineness of 400-450meshes according to the proportion of 5:3:1:1 to obtain biological phosphatic fertilizer matrix; and uniformly mixing the biocontrol bacterium seed solution and the biological phosphatic fertilizer matrix to prepare the microbial organic phosphate fertilizer capable of preventing and controlling the pseudo-ginseng blight, wherein the fertilizer contains antagonistic microbes being more than 1*1010 per unit gram, 5-6% of total nitrogen content, 6-8% of total nitrogen phosphorus and potassium and 40% of organic matters. The microbial organic phosphate fertilizer has the advantages of low cost, convenience in use, remarkable effect, and no toxicity, harmlessness and no environmental pollution, can thoroughly sterilize harmful germs in pseudo-ginseng soil, supplements nutrient elements required by growth of pseudo-ginseng plants, and improves the physical and chemical properties and microbial activity of soil with continuous cropping obstacles and stress resistance of the pseudo-ginseng plants.

Description

Technical field: [0001] The invention relates to a microbial organic phosphate fertilizer for preventing and treating Panax notoginseng wilt, belonging to the technical field of microbial organic phosphate fertilizers. Background technique: [0002] Notoginseng (Panax notoginseng (Burk.) F.H.Chen) is a plant of the genus Ginseng in Araliaceae. It is a rare and precious Chinese medicinal material unique to my country [1] and one of the most distinctive biological resources in Yunnan Province. Panax notoginseng has various pharmacological effects and health care functions such as dispelling blood stasis and stopping bleeding, reducing swelling and relieving pain, regulating blood sugar balance, anti-fatigue, anti-aging, tolerance to hypoxia, and improving immunity [2-3]. Panax notoginseng is a perennial herbaceous plant. Due to the long growth cycle of Panax notoginseng and harsh growth conditions (requiring warm winter and cool summer, no severe cold and heat, a specific envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00C05F11/08
CPCY02W30/40
Inventor 余磊赵建荣
Owner KUNMING UNIV
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