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Method for determining correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and systemic lupus erythematosus (SLE)

A technology of single nucleotide polymorphism and correlation, applied in the field of genetic engineering

Inactive Publication Date: 2014-07-16
SHENZHEN PKU HKUST MEDICAL CENT
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Problems solved by technology

But there is little evidence to support a similar association of rs4840568 with SLE in Asians, especially Han Chinese in mainland China

Method used

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  • Method for determining correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and systemic lupus erythematosus (SLE)
  • Method for determining correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and systemic lupus erythematosus (SLE)
  • Method for determining correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and systemic lupus erythematosus (SLE)

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Embodiment

[0016] 1. Research objects

[0017] 286 SLE patients (male 26 persons, 260 females, median age 29 years, age range 12-55 years) and 352 ethnically matched healthy controls (30 males, 322 females, median age 28 years, age range 17- 46 years old) participated in the study. All the healthy control group had no family history of SLE and related clinical symptoms. This study was approved by the Ethics Committee of Shenzhen Hospital and informed consent was obtained from all participants.

[0018] 2. Genotyping

[0019] Using Innogent's whole genome DNA extraction kit (Innogent, China), the collected peripheral blood genomic DNA was extracted according to the instructions of the kit. The genotype of the samples was identified by the high-resolution melting curve method of unlabeled probes. The method is briefly described as follows: first, asymmetric PCR was carried out, and the 20 mL system contained 20 ng of genomic DNA template, 1′PCR buffer solution (Takara, Japan), 200 mM ...

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Abstract

The invention discloses a method for determining the correlation between rs4840568 and rs13277113 single nucleotide polymorphisms and SLE in Chinese mainland Han people. The method of the present invention is to carry out genotyping of SLE patients through the high-resolution melting curve method of non-labeled probes, and then determine the correlation between rs4840568 and rs13277113 single nucleotide polymorphisms and SLE in Han people in mainland China. At present, it has been confirmed that environmental factors and genetic factors play an important role in the occurrence and development of SLE. Therefore, the research results of the present invention provide a theoretical basis for a deeper understanding of SLE diseases.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for determining the correlation between rs4840568 and rs13277113 single nucleotide polymorphisms and SLE in Chinese mainland Han people. Background technique [0002] Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that can affect multiple organs including skin, joints, serosa, kidney, cardiovascular system and central nervous system. The main features of this disease are the production of autoantibodies and the formation of immune complexes, as well as the consequent inflammatory response of multiple organs and tissues. Although SLE has been extensively studied, its etiology is currently only partially understood. It has been clear that environmental factors and genetic factors play an important role in the occurrence and development of SLE. Recently, a genome-wide association analysis study of the Caucasian population found several susceptibili...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 于波关明万峻张伟张超张杰杨虹陈办成
Owner SHENZHEN PKU HKUST MEDICAL CENT