Micro-fluidic chip for high-efficiency medicine screening and preparation method of micro-fluidic chip

A microfluidic chip and drug technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve problems such as no substantial breakthroughs, achieve good application prospects, reduce dosage, and simplify Effects of Cell Engraftment Process

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microfluidic chips are used to prepare microstructures and microchannels on the surface, relying on the laminar flow effect between multiple layers of liquid in the microchannels to drive sample microfluidics, and to complete the implantation of various cells at the same time, and to study and screen at the same time. The curative effect of new drugs on one type of cell and the toxicity of another type of cell have not yet had a substantial breakthrough in the application field of co-culture of multiple cells

Method used

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  • Micro-fluidic chip for high-efficiency medicine screening and preparation method of micro-fluidic chip

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip for co-culture of two kinds of cells. The cell culture solution of different cells is added from the cell solution addition hole on the left side and the cell solution addition hole on the right side respectively, and the liquid difference height between...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. The cell culture solution of different cells is added from the cell solution addition hole on the left side and the cell solution addition hole on the right side respectively, and the liquid difference height between the left and ...

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Abstract

The invention relates to a micro-fluidic chip for high-efficiency medicine screening and a preparation method of the micro-fluidic chip. Two groups of microstructures and microchannels are formed on the surface of the micro-fluidic chip; two kinds of cells are fixed in respective specific cell culture areas; after the cells are paved in the culture areas fully, a medicine to be researched are added; the effects and the influence of the same medicine on the cells in the growth process of two kinds of cells are researched intuitively under a microscope; the curative effect of the new medicine on one cell and the toxicity of the new medicine on the other cell are screened; and new thinking and new research technology are provided for large-scale medicine screening. The micro-fluidic chip is mainly applied in the related fields of cytobiology, genetics, medicine screening and the like. The micro-fluidic chip is simple in operation, realizes in-situ culture of the two kinds of cells, reduces the using amount of reagents and samples, simplifies the cell implanting process, performs medicine screening efficiently, screens the curative effect of the new medicine on one cell and the toxicity of the new medicine on the other cell, has the characteristics of portability, economy, quickness and high efficiency, and provides a new analysis technology for large-scale medicine screening.

Description

technical field [0001] The invention relates to a microfluidic chip for efficient drug screening and a preparation method thereof. There are two groups of microstructures and microchannels on the surface of the microfluidic chip. After culturing the area, add the drug to be studied, and visually study the effect and influence of the same drug on the two cells during the growth process under the microscope, and simultaneously screen the efficacy of the new drug on one cell and the toxicity of the other cell, It provides new ideas and new research technologies for large-scale drug screening, and is mainly used in related fields such as cell biology, genetics, and drug screening. Background technique [0002] In the late 1980s, in order to overcome the damage to experimenters caused by drug screening, researchers worked hard to establish a culture system that was more similar to the in vivo environment, making the in vitro environment as consistent as possible with the in vivo ...

Claims

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Application Information

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IPC IPC(8): B01L3/00
Inventor 沙俊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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