HLA (histocompatibility locus antigen) genetic typing method of HLA determinant gene through high-throughput sequencing

Abstract

The invention discloses an HLA (histocompatibility locus antigen) genetic typing method of an HLA determinant gene through high-throughput sequencing. Patterning HLA typing software based on various high-throughput sequencing platform data has important significance in clinic or biomedicine. Compared with the traditional sequencing method through a PCR-SBT (polymerase chain reaction-sequence based typing) method, the high-throughput sequencing technology has the obvious advantages in economic cost and time cost. HLA sequence data of thousands of samples can be read through an experiment and high resolution of HLA typing is achieved at one time through the high-throughput sequencing technology, and meanwhile, a new allele can be found. Qualitative leap in the aspects of flux detection, data quality, cost control and the like are achieved, 'low cost and high data' are achieved, additional economic burden of a patient caused by typing for multiple times can be avoided, the time for searching for a provider whose HLA is matched with that of the patient can also be reduced, and the precious time is saved for treating the patient.

Description

technical field

[0001] The invention relates to a gene sequencing and typing method, in particular to a high-throughput sequencing and HLA genotyping method of histocompatibility antigenic determinant genes. Background technique

[0002] HLA contains more than 200 genes, which play a key role in the human immune system. HLA is highly polymorphic, comprising about 7000 known alleles (http: / / www.ebi.ac.uk / imgt / hla / ). In bone marrow and other organ transplantation, the higher the matching degree of HLA genotype between donor and recipient, the lower the incidence of rejection, the higher the success rate of transplantation and the long-term survival rate of transplanted organs. On the contrary, the rejection reaction is more likely to occur. In a large-scale study in 2007, Stephanie J. Lee et al. analyzed 3857 transplant data recorded by the National Marrow Donors Program from 1988 to 2003 and found 8 HLA-related alleles Patients with a perfect genetic match had the highest ...

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