HLA (histocompatibility locus antigen) genetic typing method of HLA determinant gene through high-throughput sequencing

A genotyping method and antigenic determinant technology, applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., to achieve the effect of fast typing method, reduce cycle time, and avoid economic burden

Inactive Publication Date: 2013-05-01
SUZHOU JINGYIN BIOLOGICAL TECH
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Problems solved by technology

[0013] In view of the above-mentioned defects in the prior art, the purpose of the present invention is to propose a HLA genotyping method for high-throughput sequencing of histocompatibility epitope genes to solve the problems of HLA genotyping efficiency and cost

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  • HLA (histocompatibility locus antigen) genetic typing method of HLA determinant gene through high-throughput sequencing

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Embodiment Construction

[0033] The technical solution is mainly divided into two parts, which are respectively aimed at the known HLA allele types that have been included and the new HLA alleles that have not been included, and make it applicable to various high-throughput sequencing platforms.

[0034] 1) For the HLA allele types that have been included:

[0035] The standard method to determine the genotype is to compare the sequence fragments obtained by amplification sequencing to a reference database, such as IGMT / HLA (http: / / www.ebi.ac.uk / imgt / hla / ), if the sequence fragments A perfect match to a specific reference sequence in a reference database confirms that the allelic type of the tested sample is consistent with the genotype of the reference sequence. For sequence alignment, the BLASTN (http: / / blast.ncbi.nlm.nih.gov / ) nucleic acid sequence alignment tool from NCBI is preferred. The method of this HLA genotyping starts from the comparison of BLASTN. In the version 3.8.0 released on 2012-0...

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Abstract

The invention discloses an HLA (histocompatibility locus antigen) genetic typing method of an HLA determinant gene through high-throughput sequencing. Patterning HLA typing software based on various high-throughput sequencing platform data has important significance in clinic or biomedicine. Compared with the traditional sequencing method through a PCR-SBT (polymerase chain reaction-sequence based typing) method, the high-throughput sequencing technology has the obvious advantages in economic cost and time cost. HLA sequence data of thousands of samples can be read through an experiment and high resolution of HLA typing is achieved at one time through the high-throughput sequencing technology, and meanwhile, a new allele can be found. Qualitative leap in the aspects of flux detection, data quality, cost control and the like are achieved, 'low cost and high data' are achieved, additional economic burden of a patient caused by typing for multiple times can be avoided, the time for searching for a provider whose HLA is matched with that of the patient can also be reduced, and the precious time is saved for treating the patient.

Description

technical field [0001] The invention relates to a gene sequencing and typing method, in particular to a high-throughput sequencing and HLA genotyping method of histocompatibility antigenic determinant genes. Background technique [0002] HLA contains more than 200 genes, which play a key role in the human immune system. HLA is highly polymorphic, comprising about 7000 known alleles (http: / / www.ebi.ac.uk / imgt / hla / ). In bone marrow and other organ transplantation, the higher the matching degree of HLA genotype between donor and recipient, the lower the incidence of rejection, the higher the success rate of transplantation and the long-term survival rate of transplanted organs. On the contrary, the rejection reaction is more likely to occur. In a large-scale study in 2007, Stephanie J. Lee et al. analyzed 3857 transplant data recorded by the National Marrow Donors Program from 1988 to 2003 and found 8 HLA-related alleles Patients with a perfect genetic match had the highest ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王申俊其他发明人请求不公开姓名
Owner SUZHOU JINGYIN BIOLOGICAL TECH
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