Fluorescence immunochromatography flu virus detection test paper

Abstract

The invention discloses fluorescence immunochromatography flu virus detection test paper. The test paper comprises a PVC (polyvinyl chloride) bottom plate (1), and is characterized in that a connecting pad (3) is paved on the upper surface of the PVC bottom plate (1); a sample feeding hole (2) is formed in the left side of the connecting pad (3); a nitrocellulose membrane (4) and a water absorbent pad (9) are pasted at the right side of the connecting pad (3) in sequence; three line-shape or band-shaped detection antibody detection lines and beta-actin detection antibody control lines (8) are arranged on the nitrocellulose membrane (4) in sequence from left to right; the connecting pad (3) comprises a glass fiber membrane; red, green and orange fluorescein particles are uniformly attached on the glass fiber membrane; three anti-target material detection antibodies are coupled on the red fluorescein particles respectively; AMACR (alpha-methylacyl-CoA racemase) antigens are coupled on the green fluorescein particles; and beta-actin antibodies as internal references are coupled on the orange fluorescein particles. The test paper is low in price and available in materials, and has the sensitivity and the specificity of more than 95%.

Description

technical field [0001] The invention relates to a fluorescent immunochromatographic influenza virus detection test paper. Background technique [0002] Rapid and early diagnosis of various viral infections is very important for early prevention and treatment of acute infectious diseases. At present, there is no effective and rapid method for the early diagnosis of viral infection at home and abroad. The methods of molecular biology, including EILSA method, imaging diagnosis, etc., have not achieved the purpose of rapid diagnosis, so now domestic and international There is an urgent need to develop an efficient and rapid method for diagnosing various viral infections. [0003] In addition, the current detection test paper is mainly composed of PVC bottom plate, sample injection hole, binding pad, nitrocellulose membrane and water-absorbing pad. On the nitrocellulose membrane, there is only one item in the form of strips or lines to detect antibodies, which can only be used ...

AI Technical Summary

Problems solved by technology

At present, there is no effective and rapid method for the early diagnosis of viral infection at home and abroad. The methods of molecular biology, including EILSA method, imaging diagnosis, etc., have not achieved the purpose of rapid diagnosis, so now domestic and international There is an urgent need to develop an efficient and rapid method for diagnosing various viral infections

[0003] In addition, the current detection test paper is mainly composed of PVC bottom plate, sample injection hole, binding pad, nitrocellulose membrane and water-absorbing pad. On the nitrocellulose membrane, there is only one item in the form of strips or lines to detect antibodies, which can only be used It is suitable for the detection of one virus, and the detection efficiency is low, so it is not suitable for rapid detection of multiple viruses

[0004] The inventor developed a nano-influenza virus detection test strip in the early stage, which can detect three viruses at the same time, but the nano-colloidal gold composite particle material is rare and expensive

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