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Quick trenbolone residue detection test paper card and preparation method thereof

A technology for detecting test strips and trenbolone, which is applied in the field of immunochemical detection, can solve the problems of expensive and high cost, and achieve the effect of image detection, good stability, and intuitive result image

Inactive Publication Date: 2013-05-22
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are costly and require professional technicians, expensive experimental instruments and long-term preparatory work

Method used

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  • Quick trenbolone residue detection test paper card and preparation method thereof
  • Quick trenbolone residue detection test paper card and preparation method thereof
  • Quick trenbolone residue detection test paper card and preparation method thereof

Examples

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Embodiment Construction

[0027] Embodiments are described in detail with reference to the accompanying drawings. A test paper card for rapid detection of trenbolone residues, comprising a plastic box body 1 and a test paper strip 2 packaged in the plastic box body 1, the bottom layer of the test paper strip 2 is a supporting layer 3, on which the left Attached to the right are closely connected sample pads 4, gold-labeled antibody binding pads 5, nitrocellulose membranes 6, and absorbent pads 7. The plastic box body 1 is provided with a sample injection hole 8 and an observation window 9. The position of the sample hole 8 corresponds to the position of the sample pad 4 on the test strip 2, the observation window 9 corresponds to the position of the nitrocellulose membrane 6 on the test strip 2, and the nitrocellulose membrane 6 has Trenbo The detection line (T line) 11 printed by dragon-chicken ovalbumin (TRE-OVA) conjugate solution and the quality control line (C line) 10 printed by goat anti-mouse I...

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Abstract

The invention discloses a quick trenbolone residue detection test paper card and a preparation method thereof. According to the technical scheme, the quick trenbolone residue detection test paper card is characterized by comprising a plastic box body and a test strip encapsulated in the plastic box body, wherein a supporting layer is arranged on the bottom layer of the test strip, and a sample pad, a gold-labeled antibody bonding pad, a nitrocellulose membrane and an absorption pad which are closely connected with one another are sequentially attached to the supporting layer from left to right; a detection line printed by a trenbolone-albumin egg conjugate solution and a quality control line printed by a goat anti mouse IgG solution are arranged on the nitrocellulose membrane; and a colloidal-gold-labeled anti-trenbolone specific mono-clonal antibody is filled in the gold-labeled antibody bonding pad. The invention also discloses the preparation method of the quick trenbolone residue detection test paper card. The quick trenbolone residue detection test paper card has the advantages of simple operation, quickness, accuracy, high sensitivity, strong specificity, low cost and high stability, and can be used for batch detection.

Description

technical field [0001] The invention belongs to the technical field of immunochemical detection, in particular to a test paper card for rapid detection of trenbolone residues and a preparation method thereof. Background technique [0002] Trenbolone (Trenbolone, TRE), commonly known as Zhui Baolong, chemical name norandrostrienolone (17-β-hydroxyestra-4,9,11-trien-3-one-17-acetate), is a Synthetic anabolic hormone. Trenbolone can be used clinically to treat severe nutritional deficiency, osteoporosis and severe burns and other diseases. Due to its anabolic and androgenic effects, it can transfer nutrients from fat tissue to muscle tissue when it is used to feed livestock and poultry , thereby promoting muscle growth and increasing appetite. It can also increase protein synthesis, promote muscle development and red blood cell production, enhance physical strength and endurance, so it is used as a stimulant in sports games and animal competitions, which seriously violates th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/68G01N33/558G01N33/544
Inventor 姜金庆王自良张慧辉常新耀刘俊伟王淑云齐永华赵坤胡建和刘兴友
Owner HENAN INST OF SCI & TECH
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