33results about How to "Influence binding" patented technology

The present invention provides method of raising binding force of chemical coating on the surface of NdFeB permanent magnet. The NdFeB permanent magnet is pre-coated in alkaline plating solution under ultrasonic action, and by means of the activation, cavitation and stirring of ultrasonic wave, one homogeneous and compact initial deposited layer is formed on the surface of the magnet, so as to raise the binding force of chemical nickel-base coating on the surface of the magnet obviously and reduce pores in the coating. The present invention also provides the technological process including deoiling and pickling to activate before plating, the ultrasonic pre-plating and the conventional chemical plating. The formed coating has high corrosion resistance, raised binding to the substrate and perfect protection on the NdFeB permanent magnet.

The invention relates to a DNA pulldown method and a kit. The method includes the following steps of pretreating magnetic beads, wherein the magnetic beads marked by BSA(bovine serum albumin) and oligonucleotide random primer closed streptavidin are used; preparing a probe-magnetic bead compound; extracting and pretreating cell nucleus protein, wherein deoxyribonuclease is added into an extracting nucleoprotein sample for incubation, the magnetic beads are added for incubation and then removed, and supernatant is collected; carrying out pulldown; collecting the protein sample. The DNA pulldown method has the following advantages that BSA and an oligonucleotide random primer are used for closing the magnetic beads in advance, and nonspecific binding between the magnetic beads, protein and genomic DNA is reduced. Nuclease contamination is prevented through systemic nuclease protection, stability and reliability of experiments are improved, repeatability is high, and the experiment process is simplified.

The invention discloses a beta-diketone organic barium-zinc PVC heat stabilizer and a preparation method thereof, and belongs to the technical field of PVC heat stabilizers. According to the technical scheme, the beta-diketone organic barium-zinc PVC heat stabilizer is prepared through the steps that a beta-diketone organic barium compound and a beta-diketone organic zinc compound are added into dichloromethane, and after dissolution by heating is conducted, crystallization by cooling is conducted, wherein the structural formula of the beta-diketone organic barium compound is shown as follows: (please see the formula in the description), the structural formula of the beta-diketone organic zinc compound is shown as follows: (please see the formula in the description), and R represents a phenyl group. The invention further discloses the preparation method of the beta-diketone organic barium-zinc PVC heat stabilizer. The beta-diketone organic barium-zinc PVC heat stabilizer integrates the advantages of a barium stabilizer and a zinc stabilizer and the advantages of a beta-diketone compound auxiliary heat stabilizer, achieves the effect of simultaneously using the two substances and is more convenient to use.

The invention provides an ofloxacin-hemocyanin coating antigen and a preparation method thereof. The structure of the ofloxacin-hemocyanin coating antigen is shown in the description. The preparation method of the ofloxacin-hemocyanin coating antigen includes the steps that C-chain derivatization is conducted on carboxyl of ofloxacin, activation is conducted on KLH at the same time, an acid-anhydride coupling method is adopted to synthesize the coating antigen, and the prepared coating antigen has the advantages of high coupling efficiency and reactogenicity. The invention further provides a test card for testing the ofloxacin, the test card is fast in detection and high in specificity and sensitivity, the detection limit of the test card can reach 0.06 ng/ml, and the requirements of rapid detection are met at the same time.

The invention relates to a pre-plating process of a chemically-plated Ni-P alloy, in particular to an ultrasonic pre-plating process of the chemically-plated Ni-P alloy. Ultrasonic pre-plating frequency is between 40 and 50 kHz, ultrasonic pre-plating power is 100W+/-10, ultrasonic pre-plating temperature is between 56 and 60 DEG C and ultrasonic pre-plating time is between 20 and 30 minutes. A pre-plated layer obtained by the process is uniform and dense and is tightly bonded with a substrate, and the bonding force between the substrate and the plated layer can be enhanced remarkably.

A hub with an automatic pressure adjusting pack is characterized in that a rim cavity 7 is formed in a rim of a hub body 0; the pressure adjusting pack 1 is arranged in the rim cavity 7; an air nipple 6 is arranged on the pressure adjusting pack 1 and provided with an air nipple sealing pad 4 and an air nipple cap nut 5; a tension spring 2 is arranged inside the pressure adjusting pack 1; positioning skin 3 is arranged at the two ends of the tension spring 2; a rim cavity opening 8 is formed in the outer side of the rim cavity 7; a rim cavity insertion opening 9 is arranged on the upper portion of the rim cavity opening 8; a rim cavity screw hole 10 is formed in the lower portion of the rim cavity opening 8; the rim cavity opening 8 is provided with a rim cavity cover 11; the rim cavity cover 11 is provided with a cavity cover screw 12. The pressure adjusting pack with the tension spring with the setting tension is used for buffering air when the tire pressure is increased, and the tire air pressure is relieved; when the air temperature is decreased and the tire air pressure is decreased, the pressure adjusting pack release the buffered air under the action of the tension spring and corrects unstable pressure, automatic tire pressure adjustment is ensured, tire puncturing is prevented, and a vehicle runs more safely in a more oil-saving mode.

The invention provides a hot galvanizing plastic dipping coating method, and belongs to the technical field of galvanizing coating. The hot galvanizing plastic dipping coating method comprises the following steps that a silane coupling agent, water and alcohol are mixed, the pH value of an obtained mixed solution is adjusted to 4-5, and then hydrolyzing is conducted to obtain a silane coupling agent hydrolysis solution; the silane coupling agent hydrolysis solution is sprayed on the surface of a hot galvanized workpiece, then drying and preheating are conducted, and a silane film is formed on the surface of the hot galvanized workpiece to obtain a dried and preheated part; the dried and preheated part is soaked in polyethylene powder, and a plastic dipping part is obtained; and the plastic dipping part is cured. According to the hot galvanizing plastic dipping coating method, the silane coupling agent hydrolysis solution is utilized to form bonding between hot galvanizing and polyethylene interfaces, so that the binding force between the hot galvanizing and polyethylene is improved.

The invention relates to the field of biological detection, and particularly provides an acridine compound-labeled steroid hormone derivative as well as a preparation method and application thereof. An acridine compound provided by the invention is used for labeling a steroid hormone derivative, and the acridine compound and the steroid hormone derivative are connected by taking modified polyethylene glycol as a bridging substance. The acridine compound-labeled steroid hormone derivative is good in luminescence property, high in detection sensitivity, low in false positive rate, high in product yield and stable in performance.

The present invention relates to a lectin-magnetic carrier coupling complex for separating glycosylated exosomes in clinical samples, the lectin-magnetic carrier coupling complex comprising: a magnetic carrier; Lectin on the outside of the carrier. The lectin-magnetic carrier conjugate complex provided by the present invention can quickly, accurately and automatically separate glycosylated exosomes in clinical samples, with high separation efficiency, and the separated exosomes are complete in shape without rupture or fragmentation, It can be directly used for the detection of glycosylated exosome liquid, or directly for immunological related detection, or directly extract relevant nucleic acids in exosomes for nucleotide sequence detection and analysis.