Test paper card for rapidly detecting vomitoxin

A technology of vomitoxin and test paper card, which is applied in the field of immunochemical detection, can solve the problems of high cost and high cost, and achieve the effect of low cost, vivid test results and simple operation

Inactive Publication Date: 2017-07-11
HENAN ZHIWEI BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are costly and require professional technicians, expensive experimental instruments and long-term preparatory work

Method used

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  • Test paper card for rapidly detecting vomitoxin
  • Test paper card for rapidly detecting vomitoxin
  • Test paper card for rapidly detecting vomitoxin

Examples

Experimental program
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Embodiment Construction

[0071] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0072] Such as Figure 1-6 As shown, a test paper card for rapid detection of vomitoxin comprises a plastic box body 1 and a test paper strip 2 packaged in the plastic box body 1; Sample pad 4, gold standard and antibody binding pad 5, nitrocellulose membrane 6 and absorption pad 7; the nitrocellulose membrane 6 is provided with a T line 11 and a C line 10, the distance between the two is 5mm, and the T line 11 is located at On the side close to the sample pad 4, the gold label and the antibody binding pad 5 are perfused with colloidal gold-labeled anti-vomitin monoclonal antibody; the plastic box 1 is provided with a sampling hole 8 and an observation window 9 , the position of the sample injection hole 8 corresponds to the position of the sample pad 4 on the test strip 2, and the position of the observation window 9 corresponds to the position of the...

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Abstract

The invention provides a test paper card for rapidly detecting vomitoxin. The test paper card comprises a plastic box and a test strip packaged in the plastic box. The test strip comprises a bottom support layer, a sample pad, a gold label and antibody binding pad, a nitrocellulose membrane and an adsorption pad. The sample pad, the gold label and antibody binding pad, the nitrocellulose membrane and the adsorption pad are orderly fixed on the bottom support layer. The nitrocellulose membrane is provided with a T line and a C line. The colloidal gold-labeled anti-vomitoxin monoclonal antibody is poured into the gold label and antibody binding pad. The plastic box is provided with a sample filling hole and an observation window. The position of the sample filling hole is corresponding to the position of the sample pad on the test strip. The position of the observation window is corresponding to the position of the nitrocellulose membrane on the test strip. The test paper card has high sensitivity and strong specificity.

Description

technical field [0001] The invention relates to the technical field of immunochemical detection, in particular to a test paper card for rapid detection of vomitoxin. Background technique [0002] Vomitoxin, also known as deoxynivalenol (DON), is a trichothecene toxin mainly produced by Fusarium graminearum and Fusarium pink. DON may accumulate in the body to a certain extent and has no special target organs, that is, it does not cause damage to a specific organ, but it has strong cytotoxicity, which can cause intestinal dysfunction in animals, oral mucosa and dermis damage, and decreased immunity. . People and livestock will have a wide range of toxic effects after eating grains contaminated by toxins, which can cause symptoms such as vomiting, food refusal, and weight loss. The immune system and hematopoietic system are damaged, and it also has strong skin toxicity and cytotoxicity. Can damage the hematopoietic system causing death. DON is mostly distributed in grain see...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 郭芳张宝董亮张绍玲
Owner HENAN ZHIWEI BIOLOGICAL ENG
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