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Test paper card for quickly detecting residues of fluoroquinolones and preparation method of test paper card

A technology for detecting fluoroquinolones and test paper, applied in the field of immunochemical detection

Active Publication Date: 2014-11-05
HENAN ZHIWEI BIOLOGICAL ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the test strips sold in the market are used to detect a specific drug, and there is no test strip that can simultaneously detect multiple FQs veterinary drug residues

Method used

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  • Test paper card for quickly detecting residues of fluoroquinolones and preparation method of test paper card
  • Test paper card for quickly detecting residues of fluoroquinolones and preparation method of test paper card
  • Test paper card for quickly detecting residues of fluoroquinolones and preparation method of test paper card

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Embodiment Construction

[0028] Embodiments are described in detail with reference to the accompanying drawings. A test paper card for rapid detection of fluoroquinolone drug residues, comprising a plastic box body 1 and a test paper strip 2 packaged in the plastic box body 1, the bottom layer of the test paper strip 2 is a support layer 3, on which the support layer 3 From left to right, closely connected sample pads 4, gold-labeled antibody binding pads 5, nitrocellulose membranes 6, and absorbent pads 7 are attached in sequence. The plastic box body 1 is provided with a sampling hole 8 and an observation window 9. The position of the sampling hole 8 corresponds to the position of the sample pad 4 on the test strip 2, the observation window 9 corresponds to the position of the nitrocellulose membrane 6 on the test strip 2, and the nitrocellulose membrane 6 has a The test line (T line) 11 printed by norfloxacin-chicken ovalbumin (NOR-OVA) conjugate solution and the quality control line (C line) print...

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Abstract

The invention discloses a test paper card for quickly detecting residues of fluoroquinolones and a preparation method of the test paper card. The key points of the technical scheme of the test paper card are as follows: the test paper card for quickly detecting the residues of the fluoroquinolones comprises a plastic box body and a test strip which is packaged in the plastic box body, wherein the bottom layer of the test strip is used as a supporting layer; a sample pad, a gold-labeled antibody combination pad, a cellulose nitrate film and an absorption pad which are closely connected with one another are adhered on the supporting layer from left to right in sequence; a detection line which is printed by using a norfloxacin and edge albumin conjugate solution and a quality control line which is printed by using a goat anti-rat IgG solution are arranged on the cellulose nitrate film; and colloidal-gold-labeled anti-fluoroquinolone-type specific monoclonal antibodies are poured on the combination pad. The invention also discloses a method for preparing the test paper card for quickly detecting the residues of the fluoroquinolones. The test paper card is easy to operate, fast, accurate, high in sensitivity, low in cost and high in stability, and can be used for performing batch detection.

Description

technical field [0001] The invention belongs to the technical field of immunochemical detection, and in particular relates to a test paper card for rapid detection of fluoroquinolone drug residues and a preparation method thereof. Background technique [0002] Fluoroquinolones (fluoroquinolones, FQs) are a class of synthetic antibiotics developed and widely used after the 1980s. They are based on the first-generation nalidixic acid and the second-generation pipemidic acid. The 6th position of the pyridine ring introduces a fluorine atom, and the 7th position connects the piperazine ring to develop the third-generation quinolone drug, which has good tissue permeability. The mechanism of action of fluoroquinolones is to inhibit the DNA gyrase of bacteria, so that the enzyme cannot introduce nicks on the DNA double strand, destroy the metabolism and reproduction of bacteria, and achieve the purpose of killing bacteria rapidly. It is effective for Gram-positive and negative baci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N21/78G01N33/531
Inventor 姜金庆陈俊杰张晓建李桂平杨志孟晓梅原小燕栗克文周丽霞杜同亮
Owner HENAN ZHIWEI BIOLOGICAL ENG
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