Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma

A technology for primary liver cancer and precognition seeds, which is applied in the directions of drug combinations, plant raw materials, medical preparations containing active ingredients, etc., and can solve problems such as the extracts of predica seeds that have not yet been seen.

Inactive Publication Date: 2015-04-08
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the literature search, so far there is no report on the use of the extract of the seed of the oracle to inhibit the malignant proliferation of primary liver cancer cells and its direct related diseases.

Method used

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  • Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma
  • Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma
  • Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Preparation of the ethanol extract of the remaining part of Semen Semen Semen and Semen Semen Semen

[0018] Take the Chinese medicine decoction slices, and separate the seeds of the seeds; take 35g of the seeds and the rest of the non-seeds of the seeds, dry them in an oven at 60°C for 24 hours, and then crush them until the diameter of the particles is <0.25cm.

[0019] Add 350mL of 75% ethanol aqueous solution to the pulverized Zhizizi seeds and the rest of the Zhizhizifeizi granules respectively, and after soaking in airtight at room temperature for 2 hours, heat the water bath to reflux, and extract by reflux for 2 hours each time, and reflux extraction together 2 times; collect the ethanol extract, filter, concentrate to 30mL, adjust the pH to 7.0 with alkali, then add double distilled water to make up to 35mL (1g crude drug / mL).

[0020] Cell culture conditions Suction filtration sterilized, aliquoted and stored at -80°C for later use.

Embodiment 2

[0021] Example 2: Inhibition of the proliferation of primary liver cancer cells by the ethanol extracts of the seeds of Fuzhizizi and the remaining parts of Feizhizifeizi

[0022] Spread SMMC7721 liver cancer cells on a 96-well plate;

[0023] After the overnight cell attachment, the ethanol extract of the seed ethanol and the ethanol extract of the remaining part of the seed non-seed were administered at a concentration of 2.5 mg / mL; and a cytotoxic antibiotic with a concentration of 0.6 mg / mL was taken. G418 was used as a positive control.

[0024] After 24, 48, and 72 hours of administration, the proliferation and growth rate of SMMC7721 liver cancer cells were detected by MTT method, and the dose-effect curve was drawn.

[0025] figure 1 It is a comparison chart of the inhibitory effect of each drug group on the malignant proliferation of SMMC7721 liver cancer cells, in which: a is the normal cell group, b is the G418 group with a drug concentration of 0.6 mg / mL, and c i...

Embodiment 3

[0027] Example 3: Inhibition of the proliferation of primary liver cancer cells by different doses of extracts of the seeds of the pre-knowledge

[0028] Spread SMMC7721 liver cancer cells on a 96-well plate;

[0029] After the cells adhered to the wall overnight, three doses of the extracts of the seeds of the oracle with a final concentration of 1.25mg / mL, 1.88mg / mL, and 2.5mg / mL were administered respectively; and the cells with a drug concentration of 0.6mg / mL were taken The toxic antibiotic G418 was used as a positive control.

[0030] After 24, 48, and 72 hours of administration, the proliferation and growth rate of SMMC7721 liver cancer cells were detected by MTT method, and the dose-effect curve was drawn.

[0031] figure 2 It reflects the morphological effect of the medicine of the seed extract on liver cancer cells (the morphological picture observed by an inverted mirror phase microscope (400 times)); among them: a picture is conventionally cultured SMMC7721 live...

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Abstract

The invention discloses application of a fructus akebiae extract in preparation of a drug for treating primary hepatic carcinoma. The application is that the fructus akebiae extract is taken as an active ingredient to prepare a drug preparation for treating the primary hepatic carcinoma. A cancer cell growth and proliferation MTT (methyl thiazolyl tetrazolium) detection method is adopted by the invention, and the experiment demonstrates that the fructus akebiae extract has the significant effect of restraining malignant cell proliferation of the primary hepatic carcinoma, and is expected to be applied to preparation of the drug preparation for treating the primary hepatic carcinoma by restraining the malignant cell proliferation of the primary hepatic carcinoma; and a new medicinal value is opened up for the fructus akebiae extract.

Description

technical field [0001] The present invention relates to the application of an extract of Semen Semen Prognostica in the treatment of primary liver cancer, in particular, it relates to the application of the ethanol extract of Semen Prognostica in the preparation of pharmaceutical preparations for the treatment of primary liver cancer, which belongs to traditional Chinese medicine technology field. Background technique [0002] Alias: August Zha, August Zha, August Hawthorn, August Melon, August Fruit, Hundred Days Melon, Cane Melon, Waxed Melon, Lala Fruit, Mutongzi, Yajingzi, Animal Weed, Na Akebia quinata (Thunb.) Decne., Akebia trifoliate (Thunb.) Koidz. ), Akebia trifoliate (Thunb.) Koidz.var.australis ((Diels)) Rehd.) dry near-ripe fruit. Seed of Prediction is bitter and cold in nature and taste, and returns to the liver, gallbladder, stomach, and bladder meridian; soothes the liver and regulates qi, harmonizes the stomach, promotes blood circulation and relieves pain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P1/16A61P35/00A61K131/00
Inventor 方肇勤任红艳梁超张园园潘志强卢文丽刘小美
Owner SHANGHAI UNIV OF T C M
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