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Uses of different positions of Salvia miltiorrhiza Bunge and Panax Notoginseng and combinations thereof in smooth muscle cell proliferation inhibition and coronary artery restenosis control

A technique for restenosis and site, which is applied in the fields of different extraction sites of Danshen Panax notoginseng and its combination to inhibit the proliferation of smooth muscle cells and prevent and prevent restenosis after coronary artery surgery. Efficacy and other issues, to achieve the effect of reducing drug dosage

Inactive Publication Date: 2013-07-10
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, about 30% to 50% of patients will experience vascular intimal hyperplasia and restenosis 3 to 6 months after percutaneous transluminal coronary angioplasty (PTCA), which greatly affects the long-term efficacy of PTCA.

Method used

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  • Uses of different positions of Salvia miltiorrhiza Bunge and Panax Notoginseng and combinations thereof in smooth muscle cell proliferation inhibition and coronary artery restenosis control
  • Uses of different positions of Salvia miltiorrhiza Bunge and Panax Notoginseng and combinations thereof in smooth muscle cell proliferation inhibition and coronary artery restenosis control
  • Uses of different positions of Salvia miltiorrhiza Bunge and Panax Notoginseng and combinations thereof in smooth muscle cell proliferation inhibition and coronary artery restenosis control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] [Example 1] Establishment and optimization of elution methods for different elution parts of Sanqi Danshen extract.

[0033] 1. The purchase of Sanqi and Danshen herb decoction pieces (purchased from the boutique department of Dashilan Store of Beijing Tongrentang Medicinal Materials Company).

[0034] 2. Extraction and elution method of Sanqi Danshen. attached Figure 6 .

[0035] 3. Macroporous resin treatment: Soak SP825 macroporous resin with 95% ethanol for 3 days to make it fully expand, wet-pack the column, and then wash with 95% ethanol until the effluent is free from white turbidity after adding appropriate amount of water. Then wash the ethanol with distilled water until the effluent is clear of turbidity.

[0036] 4. Take 150g of Panax notoginseng and Salvia miltiorrhiza respectively, and perform ultrasonic extraction according to the method in the above figure; take another part and perform heat reflux extraction according to the above figure.

[0037]5....

Embodiment 2

[0046] [Example 2] Effects of different concentrations of Panax notoginseng and the elution site of Danshen extract on the hyperplasia of arterial intimal in SD rats.

[0047] 1. Feeding of SD rats.

[0048] A total of 82 healthy adult male SD rats, weighing 200±20 g, were purchased from the Animal Experiment Center of the Academy of Military Medical Sciences. Rats were kept in an SPF grade experimental environment.

[0049] 2. The effect of the elution site of Panax notoginseng and Salvia miltiorrhiza at the same concentration on the hyperplasia of arterial intimal in SD rats.

[0050] Preparation of animal model: intraperitoneal injection of 2% pentobarbital sodium 40mg / kg for anesthesia, after anesthesia, the rats were fixed on the surgical fixation board, the rat hair on the neck was shaved off, disinfected with iodophor and alcohol, and the neck was cut in the middle. The left artery was exposed layer by layer, the distal end of the external carotid artery was ligated, ...

Embodiment 3

[0065] [Example 3] Effects of combinations of different concentrations of Danshen Sanqi extract and different elution sites on arterial intimal hyperplasia in SD rats.

[0066] 1. Feeding of SD rats.

[0067] Fifty-four healthy adult male SD rats, weighing 200±20 g, were selected and purchased from the Animal Experiment Center of the Academy of Military Medical Sciences. Rats were kept in an SPF grade experimental environment.

[0068] 2. The effect of combination of elution fractions of Panax notoginseng and Salvia miltiorrhiza extract and its effective monomer combination on arterial intimal hyperplasia in SD rats.

[0069] Preparation of animal model: intraperitoneal injection of 2% pentobarbital sodium 40mg / kg for anesthesia, after anesthesia, the rats were fixed on the surgical fixation board, the rat hair on the neck was shaved off, disinfected with iodophor and alcohol, and the neck was cut in the middle. The left artery was exposed layer by layer, the distal end of t...

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Abstract

The invention relates to uses of different positions of Salvia miltiorrhiza Bunge and Panax Notoginseng and combinations thereof in smooth muscle cell proliferation inhibition and coronary artery restenosis control, and concretely relates to Salvia miltiorrhiza Bunge and Panax Notoginseng extraction technologies, and uses of different positions of Salvia miltiorrhiza Bunge and Panax Notoginseng and combinations thereof in vascular smooth muscle cell proliferation inhibition and coronary artery expanding formation restenosis control. Experiments prove that 50%, 95%, a 50%+95% combination and a 50%+80%+95% combination in extract products of Salvia miltiorrhiza Bunge and Panax Notoginseng can effectively inhibit the proliferation of vascular smooth muscles and control the incrassation of balloon injury postoperative endangium; and the vascular smooth muscle proliferation inhibition and balloon injury postoperative endangium incrassation control effects of the 50%+95% combination and the 50%+80%+95% combination are better than that of the individual dosing of each of the positions.

Description

technical field [0001] The present invention relates to the ultrasonic and heat reflux extraction of Danshen notoginseng, the method of carrying out alcohol-water gradient elution of the extract through SP825 macroporous resin, and the 50% elution part, 95% elution part, 50%+95% combination, 50% The +80%+95% combination and its monomer combination are used in inhibiting the proliferation of smooth muscle cells and preventing restenosis after coronary angioplasty (PTCA). Background technique [0002] Panax notoginseng is the root of the perennial herb of Araliaceae [Panax Notoginseng (Burk.) F.H.Chen]. It has the effects of promoting blood circulation, removing stasis, reducing swelling and relieving pain. Saponins are its main active ingredients, such as ginsenosides Rb1 and Rg1 . Panax notoginseng mainly contains a variety of ingredients similar to ginsenosides, such as ginsenosides Rb1, Rd, Rg1, notoginseng saponins R1, R2 and other saponins, and total saponins are its ma...

Claims

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Application Information

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IPC IPC(8): A61K36/537A61K31/704A61K31/58A61P9/00A61P9/14A61P9/10C07J73/00C07J17/00
Inventor 王升启王云飞潘从泽苗雨瑞洪婷郑冰清
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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