Stable glycated serum protein detection reagent and application thereof

Abstract

The invention provides a stable glycated serum protein detection reagent and application thereof. The reagent adopts double reagents. By storing nitroblue tetrazolium (NBT) in the neutral pH environment of a reagent R1, the problems that the NBT is easily separated out in an alkali environment, the system is turbid and the like are solved. Moreover, by adopting alkyl glycoside APG1214 serving as a new nonionic surfactant, the measurement performance is significantly improved, and a good effect on keeping the system transparent is achieved. The reagent is good in accuracy and stability, and can completely meet the clinical requirements.

Description

technical field [0001] The invention relates to a detection reagent for clinical determination of the content of glycosylated serum protein in serum and plasma and its application. Background technique [0002] Glycated serum protein (also known as fructosamine) is a non-enzymatic glycation reaction product of various proteins in serum under the action of high sugar. Serum protein glycation mainly occurs on lysine residues of protein molecules, among which albumin is the Mainly, the half-life of serum albumin is 17-19 days, and the measurement of GSP can reflect the average blood sugar level of 2 to 3 weeks before the measurement, so it is of great significance in the diagnosis of diabetes mellitus (DM) in the short-term monitoring and efficacy evaluation . [0003] At present, there are many methods for detecting GSP, but each has its own advantages and disadvantages. For example, the chromatography method has cumbersome operation steps and requires high equipment requirem...

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Problems solved by technology

[0004] In order to solve the above technical problems, the present invention provides a stable glycosylated serum protein detection reagent, the present invention is a double reagent, NBT is stored in the neutral pH environment of reagent R1, reagent R2 provides the alkaline environment required for the reaction, Thereby solving the problems such as NBT is easy to precipitate in alkaline environment and the system is turbid. The present invention uses a new non-ionic surfactant alkyl glycoside APG1214, which not only significantly improves the performance of the measurement, but also maintains the transparency of the system and prevents fat turbidity. , the effect is obvious, compared with the previously used Brij35, Tritonx-100, polyvinyl alcohol monoalkyl ether, etc., it has a better effect, and significantly improves the stability of the reagent; Human serum albumin (40g / L ) as a matrix, add an appropriate amount of pure fructosamine (Sigma) and add preservatives to make liquid standards and quality controls for the test of glycosylated serum protein, which can eliminate the influence of the matrix effect on the test to the greatest extent, and is easy to use

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