Stable glycated serum protein detection reagent and application thereof

A glycated serum protein and detection reagent technology, which is applied to stable glycated serum protein detection reagents and application fields, can solve the problems of easy precipitation of NBT and turbidity of the system, and achieves the effects of good stability, improved performance, and clinical needs.

Active Publication Date: 2013-07-10
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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Problems solved by technology

[0004] In order to solve the above technical problems, the present invention provides a stable glycosylated serum protein detection reagent, the present invention is a double reagent, NBT is stored in the neutral pH environment of reagent R1, reagent R2 provides the alkaline environment required for the reaction, Thereby solving the problems such as NBT is easy to precipitate in alkaline environment and the system is turbid. The present invention uses a new non-ionic surfactant alkyl glycoside APG1214, which not only significantly improves the performance of the measurement, but also

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  • Stable glycated serum protein detection reagent and application thereof
  • Stable glycated serum protein detection reagent and application thereof
  • Stable glycated serum protein detection reagent and application thereof

Examples

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Embodiment 1

[0028] The detection reagents for glycosylated serum protein described in this example include reagent R1, reagent R2 and their corresponding standard and quality control serum:

[0029] 1) The composition of its R1 is:

[0030] Tris-HCl buffer (pH=7.5, 25°C) 0.1mol / L

[0031] NBT (nitro blue tetrazolium chloride) 0.408g / L

[0032] Alkyl glycoside APG1214 5 g / L

[0033] Sodium cholate 1g / L

[0034] Uricase 2.5KU / L

[0035] Preservative NaN 3 0.5g / L

[0036] 2) The components of reagent R2 are:

[0037] Sodium carbonate buffer (pH=10.6, 25°C) 0.4mol / L

[0038] Preservative NaN 3 0.5g / L.

[0039] 3) Standard and quality control serum:

[0040] Add appropriate amount of pure fructosamine (Sigma) to human serum albumin (40g / L) solution prepared in normal saline and add 1g / L NaN 3 , so that the concentration of fructosamine is 292μmol / L. The m...

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Abstract

The invention provides a stable glycated serum protein detection reagent and application thereof. The reagent adopts double reagents. By storing nitroblue tetrazolium (NBT) in the neutral pH environment of a reagent R1, the problems that the NBT is easily separated out in an alkali environment, the system is turbid and the like are solved. Moreover, by adopting alkyl glycoside APG1214 serving as a new nonionic surfactant, the measurement performance is significantly improved, and a good effect on keeping the system transparent is achieved. The reagent is good in accuracy and stability, and can completely meet the clinical requirements.

Description

technical field [0001] The invention relates to a detection reagent for clinical determination of the content of glycosylated serum protein in serum and plasma and its application. Background technique [0002] Glycated serum protein (also known as fructosamine) is a non-enzymatic glycation reaction product of various proteins in serum under the action of high sugar. Serum protein glycation mainly occurs on lysine residues of protein molecules, among which albumin is the Mainly, the half-life of serum albumin is 17-19 days, and the measurement of GSP can reflect the average blood sugar level of 2 to 3 weeks before the measurement, so it is of great significance in the diagnosis of diabetes mellitus (DM) in the short-term monitoring and efficacy evaluation . [0003] At present, there are many methods for detecting GSP, but each has its own advantages and disadvantages. For example, the chromatography method has cumbersome operation steps and requires high equipment requirem...

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Application Information

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IPC IPC(8): G01N33/96G01N33/68
Inventor 陈锡良李志明甘宜梧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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