A kind of preparation method of mandarin fish otolith cross-section grinding and application thereof

Abstract

The invention discloses a manufacturing method and applications of an otolith cross section grinding sheet of a mandarin fish and belongs to the field of fish ecology. According to the manufacturing method, a sagitta serves as a research material, a cross section of the sagitta is subjected to sheet grinding, the manufactured otolith grinding sheet is placed under a microscope, the age can be identified and widths between rings can be measured through charge coupled device (CCD) software under the irradiation of incident light, ring distribution features of the otolith grinding sheet are observed, and then the age of the mandarin fish is identified. Compared with traditional identification methods, the method has the advantages that rings of the otolith of the mandarin fish are clear and visible, and an otolith nucleus is inclined towards one side of the otolith grinding sheet to form a linear nucleus area; a band area in which bright bands and dark bands are staggered is formed on the other side of the otolith starting from the otolith nucleus, bright bands are wide and are formed from spring to autumn, and dark bands are narrow and are formed in winter, so that annual rings are formed. By the aid of rings with alternating bright bands and dark bands, the age is distinguished and judged easily, so that the accuracy of identification of the age of the mandarin fish is greatly improved.

Description

technical field [0001] The invention belongs to the field of fish ecology, and in particular relates to a method for making a mandarin fish otolith cross-section grinding piece, and also relates to an application of a mandarin fish otolith cross-section grinding piece in age identification. Background technique [0002] Mandarin mandarin fish is an important freshwater economic fish in my country. It is very popular among consumers because of its fresh and tender meat, rich nutrition and no intermuscular thorns. At the same time, mandarin fish is a typical piscivorous fish in freshwater in my country, and is the top consumer in the aquatic ecosystem, playing an important role in regulating and maintaining the balance of the ecosystem. However, in recent years, due to overfishing, deterioration of the living environment, and loss of habitat and other reasons, the natural resources of mandarin fish have generally shown a downward trend. The age identification of fish is the b...

AI Technical Summary

Problems solved by technology

The entire otolith is in the shape of a flat shield, the inner and outer surfaces are uneven, the inner surface is convex outward, and the outer surface is concave inward; the core is close to the outer surface, in the middle of the dorsal and ventral lobes, and there is a A relatively obvious groove. The above-mentioned shape and structure cause great difficulties for age identification. The core of the otolith and the ring patterns around it are often not completely presented on the same grinding disc, and accurate age identification cannot be performed.

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