Nerve cell discharge performance detection method under variable concentration medicine action

A technique for the action of nerve cells and drugs, applied in the direction of material breakdown voltage, etc., can solve problems such as the inability to study the impact of nerve cell discharge

Active Publication Date: 2013-08-14
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies require a long-term observation of the changes in the firing activity of nerve cells before and after drug application, and the drug concentration range studied is only above the threshold concentration, so it is impossible to study the effect of the drug to be tested on nerve cell discharge at a concentration below the threshold.

Method used

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  • Nerve cell discharge performance detection method under variable concentration medicine action
  • Nerve cell discharge performance detection method under variable concentration medicine action
  • Nerve cell discharge performance detection method under variable concentration medicine action

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The detection method of nerve cell discharge performance under the action of variable concentration drugs, the operation is as follows

[0023] Step 1 takes the microelectrode array chip (1) with a biocompatible glass collar (2);

[0024] Step 2 Prepare the nerve cells cultured in vitro into a cell suspension of 10,000 to 100,000 cells per milliliter, then add 1 to 2 ml of the cell suspension to the biocompatible glass collar (2), and make the nerve cells Part of the cell wall structure is in contact with the microelectrodes of the microelectrode array (3), forming a bioelectrical interface;

[0025] Step 3 Use an optical microscope to observe the nerve cells on the microelectrode array of the microelectrode array chip (1), and select the two electrodes connected by the nerve cells on the microelectrode array as excitation electrodes and detection electrodes respectively, and place the microelectrode array The chip (1) is placed on the support (7) of the microelectrode...

Embodiment 2

[0036] PC12 nerve cells refer to the nerve cells differentiated from mouse adrenal pheochromocytoma cells to nerve cells after the action of nerve growth factor, and have the function of sympathetic nerve cells. This example introduces a method for detecting the discharge performance of PC12 nerve cells under the action of variable concentrations of acetylcholine.

[0037] In the first step, a cell suspension is prepared and seeded onto a microelectrode array chip. Blow off the PC12 nerve cells cultured in the cell culture bottle from the wall of the bottle with a rubber dropper, suspend them in the cell culture medium in the cell culture bottle, take out the cell suspension in the cell culture bottle and put it in In a 50ml centrifuge tube, use a 10ml pipette gun to mix the cell suspension in the centrifuge tube, then use a hemocytometer to count the cell concentration of the above cell suspension, and adjust the cell concentration of the cell suspension to 1×10 4 cells / ml,...

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Abstract

The invention discloses a nerve cell discharge performance detection method under the variable concentration medicine action. The method is characterized by comprising steps of: firstly, preparing nerve cells cultured in vitro into a cell suspension, adding the cell suspension into a biocompatible glass lantern ring of a microelectrode array chip, and contacting part cell wall structures of the nerve cells with a microelectrode of a microelectrode array so as to form a bioelectric port; adding the medicine with the concentration of 1 microgram/ml into the biocompatible glass lantern ring, putting the microelectrode array chip on a microelectrode array system support, stimulating the nerve cells through a 1mV excitation pulse signal generated by a microelectrode array (EMA) system, gradually changing the voltage amplitude of the excitation pulse signal from low to high through according to an increment of 1mV, wherein the voltage amplitude value of the excitation pulse signal is utilized when the nerve cells generate standard action potential signals as the condition threshold value of the electro-excitation signal at the current medicine concentration; and changing the medicine concentration according to the concentration gradient of 1 microgram/ml, and continuously detecting the corresponding condition threshold value of the electro-excitation signal.

Description

technical field [0001] The invention relates to a method for measuring the discharge performance of nerve cells under the action of drugs with varying concentrations below the independent threshold by using the conditional threshold of the electrical excitation signal, and in particular to a method for studying the independent threshold under the nerve signal excitation and detection system based on a microelectrode array The invention discloses a method for detecting the discharge performance of nerve cells under the action of medicines with varying concentrations, which belongs to the cross field of neuroscience and bioelectronics. Background technique [0002] The nervous system is the leading functional regulation system in the human body, and the research on neuroscience has always been a hot topic in modern scientific research. At present, the research on the effect of drugs on the nervous system is mainly carried out at three levels: molecules, cells, and animals. Amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/92
Inventor 吕晓迎王志功李贤
Owner SOUTHEAST UNIV
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