Detection method of nerve cell discharge performance under the action of variable concentration drug

Abstract

The invention discloses a nerve cell discharge performance detection method under the variable concentration medicine action. The method is characterized by comprising steps of: firstly, preparing nerve cells cultured in vitro into a cell suspension, adding the cell suspension into a biocompatible glass lantern ring of a microelectrode array chip, and contacting part cell wall structures of the nerve cells with a microelectrode of a microelectrode array so as to form a bioelectric port; adding the medicine with the concentration of 1 microgram / ml into the biocompatible glass lantern ring, putting the microelectrode array chip on a microelectrode array system support, stimulating the nerve cells through a 1mV excitation pulse signal generated by a microelectrode array (EMA) system, gradually changing the voltage amplitude of the excitation pulse signal from low to high through according to an increment of 1mV, wherein the voltage amplitude value of the excitation pulse signal is utilized when the nerve cells generate standard action potential signals as the condition threshold value of the electro-excitation signal at the current medicine concentration; and changing the medicine concentration according to the concentration gradient of 1 microgram / ml, and continuously detecting the corresponding condition threshold value of the electro-excitation signal.

Description

technical field [0001] The invention relates to a method for measuring the discharge performance of nerve cells under the action of drugs with varying concentrations below the independent threshold by using the conditional threshold of the electrical excitation signal, and in particular to a method for studying the independent threshold under the nerve signal excitation and detection system based on a microelectrode array The invention discloses a method for detecting the discharge performance of nerve cells under the action of medicines with varying concentrations, which belongs to the cross field of neuroscience and bioelectronics. Background technique [0002] The nervous system is the leading functional regulation system in the human body, and the research on neuroscience has always been a hot topic in modern scientific research. At present, the research on the effect of drugs on the nervous system is mainly carried out at three levels: molecules, cells, and animals. Amo...

AI Technical Summary

Problems solved by technology

However, these studies require a long-term observation of the changes in the firing activity of nerve cells before and after drug application, and the drug concentration range studied is only above the threshold concentration, so it is impossible to study the effect of the drug to be tested on nerve cell discharge at a concentration below the threshold.

Images