Culturing method for improving content of paclitaxel in taxus chinensis

A cultivation method and paclitaxel technology, which is applied in the field of plant light quality treatment, can solve the problems of low paclitaxel content and achieve the effects of increasing income, promoting growth, and low cost

Inactive Publication Date: 2013-09-18
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem of extremely low paclitaxel content in Taxus yunnanensis plants, the present invention provides a method for increasing the paclitaxel content in the roots of T

Method used

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  • Culturing method for improving content of paclitaxel in taxus chinensis
  • Culturing method for improving content of paclitaxel in taxus chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A. Select the seedlings of 2-year-old Taxus yunnanensis seedlings with the same source, consistent growth status, healthy root system and healthy growth;

[0019] B, the seedling that step A chooses is planted in the matrix of the following mass ratio after sterilization: river sand: deep sterile soil: forest humus=2:5:3, grows 30 days, in the meantime, by routine to each plant 30 grams of compound fertilizer containing the following elements by mass percentage is applied to the seedlings: nitrogen 46.3%, phosphorus 14.2%, potassium 7.8%, while ensuring water supply, and removing weeds in time to prevent human and animal interference and disease and insect damage. Among them, the substrate Disinfection is: use carbendazim with a mass concentration of 50% to spray poison the substrate according to the conventional amount;

[0020] C, according to the conventional amount, first spray the ground in the planting shed with green space clump clearing, and then move the seedli...

Embodiment 2

[0022] A. Select the seedlings of 2-year-old Taxus yunnanensis seedlings with the same source, consistent growth status, healthy root system and healthy growth;

[0023] B, the seedling that step A chooses is planted in the matrix of the following mass ratio after sterilization: river sand: deep sterile soil: forest humus=1:6:2, grows 20 days, in the meantime, by routine to each plant 30 grams of compound fertilizer containing the following elements by mass percentage is applied to the seedlings: nitrogen 46.3%, phosphorus 14.2%, potassium 7.8%, while ensuring water supply, and removing weeds in time to prevent human and animal interference and disease and insect damage. Among them, the substrate Disinfection is: use carbendazim with a mass concentration of 50% to spray poison the substrate according to the conventional amount;

[0024] C, by conventional amount, first use dixone powder to spray the ground in the planting shed to kill insects, then move the seedlings gained in...

Embodiment 3

[0026] A. Select the seedlings of 2-year-old Taxus yunnanensis seedlings with the same source, consistent growth status, healthy root system and healthy growth;

[0027] B, the seedling that step A chooses is planted in the matrix of the following mass ratio after sterilization: river sand: deep sterile soil: forest humus=3:4:4, grows 45 days, and in the meantime, according to the routine to each plant 30 grams of compound fertilizer containing the following elements by mass percentage is applied to the seedlings: nitrogen 46.3%, phosphorus 14.2%, potassium 7.8%, while ensuring water supply, and removing weeds in time to prevent human and animal interference and disease and insect damage. Among them, the substrate Disinfection is: use carbendazim with a mass concentration of 50% to spray poison the substrate according to the conventional amount;

[0028] C, carry out spraying and insecticide in the planting shed with green space clump clearing earlier, then the seedlings gained ...

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Abstract

The invention provides a culturing method for improving content of paclitaxel in taxus chinensis, which comprises the following steps: selecting seedlings of taxus chinensis seedlings at the age of two, which belong to the same provenance, have consistent growth conditions, have perfect roots and grow healthily; then planting the seedlings into a disinfected culture medium and enabling the seedlings to grow for 20 to 45 days; moving the seedlings into a disinfected planting shet to carry out yellow light processing for 1 to 2 years so as to obtain the taxus chinensis seedlings with high content of paclitaxel. The culturing method can obviously improve the content of paclitaxel at the roots of taxus yunnanensis seedlings and also promotes improvement of the annual growth of the seedlings to increase income. Various materials used in the culturing method have low price and can be repeatedly used, therefore, the culturing method has the characteristic of low cost. The culturing method also has the characteristics of simplicity for operation, easiness for mastering the method, high income and the like.

Description

technical field [0001] The invention relates to a method for improving the content of paclitaxel in the roots of Taxus yunnanensis seedlings by utilizing yellow light, and belongs to the technical field of plant light quality treatment. Background technique [0002] Light is one of the essential environmental factors for plant growth, and it is the source of plant photosynthesis energy and growth and development signals, and affects the growth and development, morphological structure, physiology and biochemistry of plants. In terms of physiological and biochemical effects, light mainly affects the secondary metabolism of plants, especially the synthesis and content of plant secondary metabolites. Plant secondary metabolites are important sources of medicines, spices and industrial raw materials, and have an important impact on human production and life. For example, the secondary metabolite of Taxus yunnanensis, paclitaxel, is the main natural anticancer drug. Therefore, ex...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06
Inventor 刘万德张志钧苏建荣臧传富李帅锋郎学东缪迎春
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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