Detection method for CVA16 in environmental water body

A CVA16-R and detection method technology, which is applied in the field of environmental water quality detection, can solve the problems of complex detection influencing factors, no CVA16 detection method, and incapable of accurate qualitative or quantitative detection of CVA16
CN103305629BInactive Publication Date: 2015-07-15XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
Publication Date
2015-07-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detection method for CVA16 in an environmental water body, wherein a semi-nested RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and real-time fluorescence quantification RT-PCR reaction system is established to detect CVA16 virus in the environmental water body by using a recombinant plasmid as a reference product, and the recombinant plasmid is prepared by using a primer and a probe aiming at CVA16 virus designed according to a genetic conservative fragment of enterovirus belonging to a VP1 serotype area. The method has good specificity and high sensitivity and precision, and can qualitatively and quantitatively detect CVA16 virus in the environmental water body accurately.
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Description

technical field

[0001] The invention belongs to the technical field of detection of environmental water quality, and in particular relates to a detection method of CVA16 virus in environmental water. technical background

[0002] Coxsackievirus A16 (CVA16) is one of the main pathogens causing HFMD and belongs to the Enterovirus genus. CVA16 is a water-borne virus that can be released into environmental waters from the secretions of CVA16-infected patients and can survive in environmental waters for a long time. The water body polluted by it becomes an important pollution source of CVA16, which can cause the outbreak of hand, foot and mouth disease.

[0003] The existing methods for detecting CVA16 mainly include cell culture method and polymerase chain reaction (Polymerase Chain Reaction, PCR). There are a variety of pathogenic microorganisms in environmental water, especially other enteroviruses. These microorganisms interfere with the detection of CVA16 by cell culture, ...

Claims

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