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Method for establishing mice transplantable breast cancer experimental pulmonary metastasis model

A metastasis model, breast cancer technology, applied in the direction of tumor/cancer cells, vertebrate cells, animal husbandry, etc., can solve the problems of high cost, low incidence of metastasis, and long tumor formation time

Inactive Publication Date: 2013-09-25
BENGBU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Breast cancer transplantation metastasis models can be divided into two types: experimental metastasis models and spontaneous metastasis models. At present, breast cancer metastasis models are mostly spontaneous metastasis models, mainly through the spontaneous metastasis of primary tumors in diseased mice. The incidence of spontaneous tumor metastasis is 70-80%, but the spontaneous metastasis model of breast cancer has the disadvantages of low incidence of metastasis, long time for tumor formation, and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Establish a mouse transplantable breast cancer experimental lung metastasis model as follows:

[0023] a) Spontaneous breast cancer tumors in mice were excised under aseptic conditions, and washed 4 times with sterile phosphate buffered saline;

[0024] b) Grind the tumor mass in a 400-mesh mesh sieve, place the petri dish for collection under it, rinse the ground cells with sterile phosphate buffer solution, centrifuge twice at 2500r / min for 5min, and the cells sink to the bottom ;

[0025] c) Counting cells, take 10 μL of cells and drop them on the counting plate, calculate according to the calculation formula (counting up does not count down, counting left does not count right), and adjust the cell concentration to 1×10 with DMEM culture medium 7 cell / mL;

[0026] d) Select 20 C from Group A 3 For H mice, inject 0.3 mL of tumor cell suspension into the tail vein of each mouse with a sterile syringe;

[0027] e) Feed under normal conditions, and reproduce normally...

Embodiment 2

[0030] Establish a mouse transplantable breast cancer experimental lung metastasis model as follows:

[0031] a) Spontaneous breast cancer tumors in mice were excised under sterile conditions, and washed 3 times with sterile phosphate buffered saline;

[0032] b) Grind the tumor mass in a 300-mesh mesh sieve, place the petri dish for collection under it, wash the ground cells with sterile phosphate buffer solution, centrifuge twice at 2500r / min for 5min, and the cells sink to the bottom ;

[0033] c) Count the cells, take 10 μL of cells and drop them on the counting plate, calculate according to the calculation formula (counting up does not count down, counting left does not count right), and adjust the cell concentration to 2×10 with DMEM culture medium 7 cell / mL;

[0034] d) Select 20 C from group B 3 For H mice, inject 0.1 mL of tumor cell suspension into the tail vein of each mouse with a sterile syringe;

[0035] e) Feed under normal conditions, and reproduce normally...

Embodiment 3

[0038] Establish a mouse transplantable breast cancer experimental lung metastasis model as follows:

[0039] a) Spontaneous breast cancer tumors in mice were excised under aseptic conditions, and washed 4 times with sterile phosphate buffered saline;

[0040] b) Grind the tumor mass in a 400-mesh mesh sieve, place it on a petri dish for collection, rinse the ground cells with sterile phosphate buffer, centrifuge twice at 2500r / min for 5min, and the cells sink to the bottom ;

[0041] c) Count the cells, take 10 μL of cells and drop them on the counting plate, calculate according to the calculation formula (counting up does not count down, counting left does not count right), and the cell concentration is adjusted to 1.5×10 with DMEM culture medium. 7 cell / mL;

[0042] d) Select 20 C from Group C 3 For H mice, inject 0.2 mL of tumor cell suspension into the tail vein of each mouse with a sterile syringe;

[0043] e) Feed under normal conditions, and reproduce normally for ...

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Abstract

The invention provides a method for establishing a mice transplantable breast cancer experimental pulmonary metastasis model. The method comprises the following steps: grinding mice spontaneous breast cancer adenoma in a mesh screen, carrying out centrifugal precipitation, preparing a tumour cell suspension with the cell concentration of (1-2)*107 cell / mL by the use of a nutrient solution, carrying out tail intravenous injection on each mouse by the use of 0.1-0.3mL of the tumour cell suspension, feeding the mice under normal conditions and normally breeding for 25-35 days to finish the pulmonary metastasis model. The injected tumor cells are prepared by grinding genuine C3H mice tumor cells in mice, thus better simulating the in vivo metastasis process. According to the breast cancer experimental metastasis model, tumour cells are directly injected into animal bodies through a tail intravenous injection mode; after the injection, animals are raised under normal conditions without special environment; and after transplantation, it is detected that metastatic lesions are formed in C3H mice lungs through blood circulation. The method provided by the invention has advantages of high incidence rate, short tumour formation time, high incidence rate of metastasis and low cost, etc.

Description

technical field [0001] The invention relates to a method for establishing a cancer cell metastasis model, in particular to a method for establishing a mouse transplantable breast cancer experimental lung metastasis model. Background technique [0002] Tumor metastasis is the main cause of death for more than 90% of cancer patients, so blocking metastasis is an important means of survival for cancer patients. Since the lung tissue is rich in blood and oxygen supply, it is an organ that is easily invaded by malignant tumors. Blocking lung metastasis is of great significance to the treatment of breast cancer, because many studies on tumor diseases cannot be carried out directly in tumor patients, but can be done with the help of experimental animals, usually through C 3 H mice for live animal experiments (C 3 H mice are not immunodeficient compared to immunodeficient mice). [0003] To study the molecular mechanism of breast cancer metastasis and further provide a better expe...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027
Inventor 刘浩蒋志文蒋琛琛晁振华蒋玉敏
Owner BENGBU MEDICAL COLLEGE
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