Metarrhizum anisopliae for preventing and treating palm insect rhynchophorus ferrugineus and application thereof

A technology of red palm weevil and metarhizium anisopliae, applied in the field of microorganisms, to achieve good insecticidal effect, reduce losses, and good insecticidal effect

Inactive Publication Date: 2013-11-13
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Metarrhizum anisopliae for preventing and treating palm insect rhynchophorus ferrugineus and application thereof
  • Metarrhizum anisopliae for preventing and treating palm insect rhynchophorus ferrugineus and application thereof

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Example Embodiment

[0014] Example 1: Screening and identification of strains

[0015] 1. Screening of strains

[0016] Take the collected samples of red palm weevil worms, disinfect them with 70% alcohol for one minute, wash with sterile water, and then disinfect the infected samples with 0.1% mercuric chloride for 30 seconds, and wash them with sterile water 3 times. Pick a small amount of dead worm tissue and inoculate it in the separation SDAY medium (4% glucose, 1% peptone, 1% yeast powder, 2% agar and 500μg / mL streptomycin), inverted at 28±1℃, inverted and cultured for 3-4 days , Select monospores to inoculate purified SDAY medium (4% glucose, 1% peptone, 1% yeast powder and 2% agar), 3 points per dish, arranged in a triangle, and obtain insecticidal strains after purification by monospores , Named Metarhizum anisopliae WENCHANG-LV2, has been deposited in the General Microbiology Center of China Microbial Culture Collection and Management Committee on June 9, 2013, Address: No. 3, No. 1, Beiche...

Example Embodiment

[0024] Example 2 Indoor insecticidal activity of strains against red palm weevil

[0025] Take the isolated, purified and cultured Metarhizum anisopliae WENCHANG-LV2 strain, put the conidia powder into a 10ml sterile centrifuge tube, add 5mL 0.1% Tween 80 sterile water, and vortex. Shake the device for 15 minutes to mix, and then use a hemocytometer to measure the number of spores per milliliter of suspension. After appropriate adjustments, the strain is configured to a spore concentration of 1.0×10 8 Pieces / mL, store in a refrigerator at 4°C for later use. The spores of the WENCHANG-LV2 strain of Metarhizum anisopliae were prepared into 5 concentrations of oil by a gradient dilution method, the concentrations were 1.0×10 8 , 5×10 7 , 2.5×10 7 , 1.25×10 7 , 6.25×10 6 Pcs / ml. The preparation method of the spore oil suspension is: scrape the conidia powder of the strain, add the mixed oil at a ratio of 10:1 of palm oil and refined coconut oil, and mix with a vortex shaker for 15 mi...

Example Embodiment

[0028] Example 3 Outdoor control effect of strains

[0029] Bring the oil agent of Metarhizum anisopliae WENCHANG-LV2 strain to the field, and dilute with the mixed oil to contain 1×10 conidia 8 Pieces / mL, and stir evenly, spray with ultra-low volume.

[0030] Spray prevention and control started on May 6, 2013. At that time, the weather was fine, the temperature was 34°C, and the wind was breeze. When spraying, the crosswind angle was 50°. The fog droplets were distributed more and evenly on the tree head (near the growth point), and the coverage density was even. 23.8 drops / cm 3 . Check once a week, select 3 coconut trees with the same damage condition each time for anatomy, count the number of live insects and infected dead insects, the results are shown in Table 2. It can be seen from the results that after 15 days of treatment, the survival rate of the larvae in the trunk began to decrease significantly, and the survival rate was only 41.25% after 40 days, indicating that Met...

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Abstract

The invention relates to metarrhizum anisopliae for preventing and controlling palm insect rhynchophorus ferrugineus and application thereof. The strain is named metarrhizum anisopliae WENCHANG-LV2 is preserved in the China General Microbiological Culture Collection Center on June 9th, 2013 with the preservation number of CGMCC NO.7692. The metarrhizum anisopliae WENCHANG-LV2 provided by the invention can be applied to rhynchophorus ferrugineus prevention and control. Direct application testing indicates that the metarrhizum anisopliae WENCHANG-LV2 has a good insecticide effect, can be used for effectively controlling the number of species of rhynchophorus ferrugineus, is a biocontrol strain which has a potential meaning in preventing and treating rhynchophorus ferrugineus, and favors the development of palm industry in China.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a Metarhizium anisopliae strain and its application, in particular to a Metarhizium anisopliae strain for preventing and treating the palm pest, Ruthia anisopliae, and its application. Background technique [0002] Red Palm Weevil (Rhynchophorus ferrugineus Oliv.) belongs to Coleoptera (Coleoptera) Weevil family (Curculionidae) Palm Weevil genus Rhynchophorus sp., the English name is Red Palm Weevil (RPW for short), also known as Asian palm weevil, rusty palm Elephant, coconut beetle, cryptorhynchus, Indian red palm weevil, etc. The red palm weevil is mainly distributed in Taiwan, Fujian, Hong Kong, Hainan, Guangdong, Guangxi, Shanghai, Yunnan, Tibet (Medog) and other places in China. The results of the pest risk analysis of the red palm weevil show that it is a high-risk pest and is easy to colonize in many areas of China. It poses a great threat to my country's oil palm, areca nut,...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 孙晓东覃伟权刘丽钟宝珠李朝绪张晶
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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