Method for constructing pharmacological mechanism of Jinlong capsule
A technology of Jinlong capsule and pharmacology, which is applied in the fields of traditional Chinese medicine and systems biology, can solve the problems of single research method of pharmacology and efficacy, etc.
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Embodiment 1
[0053] Fifty-five female nude mice aged 5-6 weeks were taken, and the U87-RFP tumor tissue obtained from the subcutaneous tissue of syngeneic mice was cut into 1mm by surgical orthotopic transplantation. 3 Using surgical in situ inoculation technique, the tumor mass was planted into the nude mouse skull, and U87 cells were transfected by constructing a red fluorescent protein-labeled plasmid, and then the U87 cells were injected into the brain to establish an orthotopic brain tumor model. Orthotopic mouse model of human brain tumors expressing red fluorescent protein (U87-RFP).
Embodiment 2
[0055] 96mg / kg Temozolomide, 0.75g / kg Jinlong Capsules, 1.5g / kg Jinlong Capsules and the blank group were administered respectively for 28 consecutive days. The FluorVivo Model-100 fluorescence imager was used for non-invasive real-time imaging observation, and the tumor volume and weight were recorded.
[0056] On the 15th day after administration of 96 mg / kg Temozolomide, 0.75 g / kg Jinlong Capsules and 1.5 g / kg Jinlong Capsules, fluorescence in vivo imaging was performed to observe the size of intracranial tumors in nude mice. like figure 1 As shown, 96mg / kg temozolomide, 0.75g / kg Jinlong capsule and 1.5g / kg Jinlong capsule have different degrees of inhibition compared with the model group.
[0057] Continuous administration of 96 mg / kg Temozolomide, 0.75 g / kg Jinlong Capsules and 1.5 g / kg Jinlong Capsules was administered for 28 days, and intracranial tumor tissues of nude mice were taken for weighing on the 28th day. like figure 2 As shown, compared with the model grou...
Embodiment 3
[0059] Take 3 brain tumor tissues of the blank control group and 3 brain tumor tissues of the 0.75g / kg Jinlong Capsule group, extract the total RNA of the samples, and use RT-PCR method to reverse transcribe and synthesize cDNA (using RT-PCR kit, Invitrogen Company), Biotin-labeled cRNA was synthesized by in vitro transcription, and the cRNA was purified. After the cRNA was fragmented, it was hybridized with the Human Genome U133Plus2.0 gene chip from Affymetrix.
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