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Method for constructing pharmacological mechanism of Jinlong capsule

A technology of Jinlong capsule and pharmacology, which is applied in the fields of traditional Chinese medicine and systems biology, can solve the problems of single research method of pharmacology and efficacy, etc.

Active Publication Date: 2013-11-20
BEIJING JIANSHENG PHARMA
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Problems solved by technology

[0009] For this reason, the technical problem to be solved by the present invention is that the pharmacological and pharmacodynamic research means of Chinese medicine, especially compound Chinese medicine, is too single in the prior art, and then provide a A method for constructing the pharmacological mechanism of Jinlong Capsules by means of omics and systems biology

Method used

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  • Method for constructing pharmacological mechanism of Jinlong capsule
  • Method for constructing pharmacological mechanism of Jinlong capsule
  • Method for constructing pharmacological mechanism of Jinlong capsule

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Experimental program
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Effect test

Embodiment 1

[0053] Fifty-five female nude mice aged 5-6 weeks were taken, and the U87-RFP tumor tissue obtained from the subcutaneous tissue of syngeneic mice was cut into 1mm by surgical orthotopic transplantation. 3 Using surgical in situ inoculation technique, the tumor mass was planted into the nude mouse skull, and U87 cells were transfected by constructing a red fluorescent protein-labeled plasmid, and then the U87 cells were injected into the brain to establish an orthotopic brain tumor model. Orthotopic mouse model of human brain tumors expressing red fluorescent protein (U87-RFP).

Embodiment 2

[0055] 96mg / kg Temozolomide, 0.75g / kg Jinlong Capsules, 1.5g / kg Jinlong Capsules and the blank group were administered respectively for 28 consecutive days. The FluorVivo Model-100 fluorescence imager was used for non-invasive real-time imaging observation, and the tumor volume and weight were recorded.

[0056] On the 15th day after administration of 96 mg / kg Temozolomide, 0.75 g / kg Jinlong Capsules and 1.5 g / kg Jinlong Capsules, fluorescence in vivo imaging was performed to observe the size of intracranial tumors in nude mice. like figure 1 As shown, 96mg / kg temozolomide, 0.75g / kg Jinlong capsule and 1.5g / kg Jinlong capsule have different degrees of inhibition compared with the model group.

[0057] Continuous administration of 96 mg / kg Temozolomide, 0.75 g / kg Jinlong Capsules and 1.5 g / kg Jinlong Capsules was administered for 28 days, and intracranial tumor tissues of nude mice were taken for weighing on the 28th day. like figure 2 As shown, compared with the model grou...

Embodiment 3

[0059] Take 3 brain tumor tissues of the blank control group and 3 brain tumor tissues of the 0.75g / kg Jinlong Capsule group, extract the total RNA of the samples, and use RT-PCR method to reverse transcribe and synthesize cDNA (using RT-PCR kit, Invitrogen Company), Biotin-labeled cRNA was synthesized by in vitro transcription, and the cRNA was purified. After the cRNA was fragmented, it was hybridized with the Human Genome U133Plus2.0 gene chip from Affymetrix.

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Abstract

The invention belongs to the traditional Chinese pharmacology field, and concretely relates to a method for constructing the pharmacological mechanism of a Jinlong capsule through a biological and genetic engineering process. The method analyzes the system biology of a processed gene chip result and constructs the pharmacological mechanism of the Jinlong capsule by utilizing a Thomson Reuters Genego database through the hybridization, detection and analysis of the gene chip via omics and the system biology process. The invention also discloses a use of the Jinlong capsule as a brain tumor resistance medicine and in chemotherapeutic drug tolerance activity reversion. And the pharmacological mechanism of the Jinlong capsule as the brain tumor resistance medicine and in the chemotherapeutic drug tolerance activity reversion is constructed through the method.

Description

Technical field [0001] The present invention is a method of Chinese medicine and system biology, which involves a method of building a pharmacological mechanism for using biological and genetic engineering means. Background technique [0002] Traditional Chinese medicine is Chinese medicine. It is a unique medicine for traditional Chinese medicine in China. It has a long history and has a significant effect in clinical use of thousands of years.Fresh medicine is an important part of traditional Chinese medicine. Because it is rich in natural activity ingredients, it is highly respected in preventing and controlling certain diseases, especially critical illness.Traditional fresh medicines are often used and used, and their clinical use is limited due to environmental and regional factors.With the success of the "low temperature and frozen modern biochemical separation extraction technology", the "modern fresh medicine" with the characteristics of modern dosage forms came into bein...

Claims

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Application Information

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IPC IPC(8): A61K49/00C12Q1/68C12Q1/02A61K35/58A61K9/48A61P35/00A61K35/56A61K35/583
Inventor 黄卉曲育莹岳贵娟李建生
Owner BEIJING JIANSHENG PHARMA
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