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Test paper card for rapidly detecting flumequine and preparation method thereof

A technology for detecting test paper and flumequine, which is applied in the field of rapid detection of flumequine test paper card and preparation, which can solve the problems that the effectiveness of food safety monitoring cannot be guaranteed, the quality requirements of testing personnel are high, and the illegal use of flumequine is difficult, etc. Achieve the effect of easy promotion, easy promotion and application, and wide application range

Active Publication Date: 2013-12-18
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection time of these methods is generally long, the detection is inconvenient, the detection cost is high, and the quality requirements of the detection personnel are high, which seriously restricts the application in actual detection and is difficult to popularize
Due to the limitations and severe hysteresis of these methods, it is difficult to monitor the illegal use of flumequine, and the effectiveness of food safety monitoring cannot be guaranteed

Method used

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  • Test paper card for rapidly detecting flumequine and preparation method thereof
  • Test paper card for rapidly detecting flumequine and preparation method thereof
  • Test paper card for rapidly detecting flumequine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Flumequine rapid detection test paper card, see figure 1 , figure 2 , including the shell and the test paper core located in the shell, in the figure bottom plate 1, sample pad 2, colloidal gold marking pad 3, detection reaction area 4, invisible detection imprint 5, invisible control imprint 6 and water-absorbing pad 7 jointly form the test paper core; test paper The material of the card shell is engineering plastics, and the base 12 is provided with a fixing groove 11 for the test paper core; the sample injection hole 8 on the panel 10 corresponds to the sample pad 2 of the test paper core; the panel 10 is provided with a groove 13 combined with the base , the detection window 9 on the panel 10 corresponds to the detection reaction area 4 of the test paper core, and is a window for observing and judging results, with T and C printed beside it, respectively corresponding to the invisible detection imprint 5 and the invisible control on the detection reac...

Embodiment 2

[0051] Embodiment two: detection test paper card structure is basically the same as embodiment one, the difference is: replace anti-flumequine polyclonal antibody with anti-flumequine monoclonal antibody, prepare flumequine colloidal gold marker and colloidal gold marker pad The carrier protein coupled with flumequine is chicken ovalbumin OVA, and the rabbit anti-mouse IgG antibody solution is replaced with goat anti-rabbit IgG to prepare a control blot on the detection reaction area. Stealth detection blots and stealth control blots are arranged as " ＋ "or"··".

[0052] Others including test samples, operation methods and result judgments are the same as those in Example 1 and will not be repeated.

Embodiment 3

[0053] Embodiment 3: The structure of the detection test paper card is basically the same as that of Embodiment 1, the difference is that:

[0054] The bottom plate is made of rigid plastic strips; the sample pad is made of nylon membrane; the detection reaction area is made of pure nitrocellulose membrane; the flumequine carrier protein is chicken ovalbumin; the invisible control blot is printed with goat anti-mouse IgG antibody solution system. Others including test samples, operation methods and result judgments are the same as those in Example 1 and will not be repeated.

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Abstract

The invention relates to a test paper card for rapidly detecting flumequine and a preparation method thereof. The test paper card comprises a shell and a test paper core, wherein the test paper core comprises a bottom plate, and a sample cushion, a colloidal gold label cushion, a detection reaction region and a water absorption cushion which are arranged on the bottom plate; the colloidal gold label cushion is adsorbed with a specific antibody of flumequine; the detection reaction region is provided with a detection print printed by a flumequine coupling carrier protein solution and a contrast print printed by a goat or (rabbit) anti-mouse IgG (Immunoglobulin G) solution or a goat anti-rabbit IgG solution; the specific antibody of flumequine is a colloidal gold labeled flumequine monoclonal antibody or polyclonal antibody. In the preparation process, a base and a panel are embedded together; a detection window on the panel corresponds to the detection reaction region of the test paper core; a sample feeding hole corresponds to the sample cushion of the test paper core. The test paper card is strong in specificity and high in sensitivity, is simple, convenient and rapid to detect and is strong in timeliness; the result display is visual, direct and accurate; the detection cost is low and an applicable range is wide; therefore, the test paper card is convenient to popularize.

Description

technical field [0001] The invention relates to a device for detecting flumequine, in particular to a test paper card for rapidly detecting flumequine and a preparation method thereof. technical background [0002] Flumequine is a fluoroquinolone antibiotic with broad antibacterial properties, strong bactericidal activity, and wide distribution in the body, and has no cross-resistance with other antibacterial drugs. At present, it is widely used in livestock and poultry and aquaculture. As a drug used in food animals, flumequine has attracted much attention for its residual carcinogenic toxicity in animal edible tissues and products. The country has made strict regulations on the maximum residues in different meat products. Because of flumequine's low price, wide range of antibacterial properties, and strong bactericidal activity, farmers blindly pursue the effect of prevention and treatment, artificially adding excessive amounts, resulting in the widespread phenomenon of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/566
Inventor 王方雨张改平刘珍胡骁飞李青梅滕蔓邢广旭
Owner HENAN ACAD OF AGRI SCI
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