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Rapid breeding method for grape seedlings

A grape seedling and rapid technology, applied in the fields of botany equipment and methods, horticulture, soilless cultivation, etc., can solve the problems of long cutting propagation cycle, difficulty in mass production, and high cost, and achieve improved breeding speed and breeding survival rate, Realize the effect of automation, precision and efficiency

Inactive Publication Date: 2014-01-15
镇江市丹徒区紫杉生态农业园
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AI Technical Summary

Problems solved by technology

However, the cutting propagation cycle is long, the survival rate is low, and it is difficult to achieve large-scale seedling cultivation.
Tissue culture technology is used to cultivate non-toxic seedlings (first-generation seedlings), which are only suitable for scientific experiments, and are expensive and difficult to produce on a large scale

Method used

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Embodiment Construction

[0013] The rapid breeding method of the grape seedling of the embodiment of the present invention comprises the following steps:

[0014] A. Selection and pretreatment of in vitro materials: Select the new branches of the year with good growth and no disease on the grape branches, intercept the branches with 1-2 spores, make the lower incision at an angle of 45 degrees, and use 1000 times diluted water for the incision. Disinfect with carbendazim, then soak for 30 minutes with NAA diluted 500 times with water;

[0015] B. Colonization of isolated materials: insert the aforementioned pretreated isolated materials into the perlite matrix, and the isolated materials penetrate into the matrix by 3 to 5 cm, subject to non-overlapping;

[0016] C. Rooting period management of isolated materials: light intensity 2500Lux, light time 12 hours / day, substrate substrate and air temperature, day temperature 28°C, night temperature 19.5°C, substrate relative humidity 62%, air relative humid...

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Abstract

The invention belongs to the plant non-test-tube rapid breeding technology, and relates to a rapid breeding method for grape seedlings. The method comprises the following steps: A. selecting and pre-treating an ex-vivo material: selecting stem buds from sprouts on stolons of the grape seedlings, disinfecting the notch through carbendazim, and soaking through naphthylacetic acid; B. permanently planting the ex-vivo material: inserting the ex-vivo material into a pearlstone substrate; C. performing the rooting period management of the ex-vivo material: controlling the plant growth environment condition through an automatic control system; if new roots begin to bud, spraying a nutrient solution once per 24 hours, and obtaining the length of the new root is 3 to 5 cm through visual-estimating; D. performing seedling refining period management of the ex-vivo material: detecting that 1 to 2 pieces of blades are grown at the upper part of the ex-vivo material, and then ensuring the ex-vivo material to enter a nursery stock period; E. performing nursery stock period management: transplanting the ex-vivo material into a culture container or a culture ground bed with conventional grape culture soil, and obtaining commodity seedlings after 18 to 25 days growing. The method greatly improves the breeding speed and breeding survival rate, and is suitable for industrialized implementation of large scale rapid breeding better.

Description

technical field [0001] The invention belongs to the non-test tube rapid propagation technology of plants, and relates to a rapid propagation method of grape seedlings. Background technique [0002] Existing grape seedling breeding methods mainly contain tissue culture technology and traditional branch cutting technology. However, the cutting propagation cycle is long, the survival rate is low, and it is difficult to achieve large-scale seedling cultivation. Tissue culture technology is used to cultivate non-toxic seedlings (first-generation seedlings), which are only suitable for scientific experiments, and are expensive and difficult to produce on a large scale. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a rapid breeding method for grape seedlings that can greatly increase the breeding speed and breeding survival rate and is suitable for large-scale seedling cultivation. [0004] The rapid breeding method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00
Inventor 夏彬
Owner 镇江市丹徒区紫杉生态农业园
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