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Preparation method and applications of fusarium oxysporum inoculant

A technology of Fusarium oxysporum and inoculum, applied in the field of biopharmaceuticals, can solve the problems of unsuitability for large-scale production, poor root-knot nematode effect, high economic cost, etc., and achieves the effects of low price, reduced dosage, and guaranteed activity

Inactive Publication Date: 2015-05-06
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

But the preparation method of the Fusarium oxysporum inoculant of this control cucumber root-knot nematode at present often uses a large amount of thiazolyl phosphine to cooperate to make, and using a large amount of thiazolyl phosphonium will bring very high economic cost, is not suitable for large-scale Production, the effect of controlling root-knot nematode is poor

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  • Preparation method and applications of fusarium oxysporum inoculant
  • Preparation method and applications of fusarium oxysporum inoculant

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preparation example Construction

[0021] The invention provides a preparation method of Fusarium oxysporum inoculum, the preparation method comprising the following steps:

[0022] (1) Strain cultivation: Purify the non-pathogenic Fusarium oxysporum inside the cucumber root system as the parent fungus, and sterilize the fungus at 121 °C for 20-30 minutes at 25-28 °C Cultivate 3-5 days in the plate culture medium after;

[0023] (2) Fermentation broth preparation: After the liquid medium is sterilized by high pressure and damp heat, add the Fusarium oxysporum cultured in step (1), and then culture it with stirring at a temperature range of 25-28°C for 7-10 days. Obtain the Fusarium oxysporum liquid after culturing as the fermentation liquid;

[0024] (3) Fermentation culture: put the solid medium into the fermentation bottle, and sterilize it at 121°C for 60-90 minutes, then add the fermentation broth generated in step (2), mix well, and Fermentation and cultivation for 7-10 days at a temperature range of 25-...

Embodiment 1

[0037]Embodiment 1: the mycelium of Fusarium oxysporum bacterial strain fus-1 is inoculated on plate medium, and medium formula is PDA medium, and this medium comprises potato 200g, glucose 20g and agar 17g, and configures with distilled water, set up to 1000 mL. After the medium was sterilized at 121°C for 20 minutes, Fusarium Fus-1 was inoculated and cultured at 25°C for 3 days to obtain activated strains.

[0038] The activated fus-1 strain was inoculated into 250 ml Erlenmeyer flasks, each containing 125 ml of PDA liquid medium, and cultured at 25°C for 7 days at a stirring speed of 170 r / min to obtain a large amount of mycelium and Sporulation. The formula of the liquid medium is 200g of potatoes and 20g of glucose, which are prepared with distilled water and adjusted to 1000 mL.

[0039] Put the above fermented liquid into the solid medium, mix well, ferment and cultivate at 25°C for 7 days, the solid medium is covered with white hyphae and spores. The solid medium co...

Embodiment 2

[0041] Embodiment 2: Inoculate the mycelium of Fusarium oxysporum strain fus-1 on the plate culture medium, the medium formula is PDA medium, and this medium comprises potato 200g, glucose 20g and agar 17g, and configures with distilled water, set up to 1000 mL. After the medium was sterilized at 121° C. for 30 minutes, Fusarium Fus-1 was inoculated and cultured at 28° C. for 5 days to obtain activated strains.

[0042] The activated fus-1 strain was inoculated into 250 ml Erlenmeyer flasks, each containing 125 ml of PDA liquid medium, and cultured at 28°C for 10 days at a stirring speed of 220 r / min to obtain a large amount of mycelium and branch Sporulation. The formula of the liquid medium is 200g of potatoes and 20g of glucose, which are prepared with distilled water and adjusted to 1000 mL.

[0043] The fermented liquid propagated by the liquid is added to the solid medium, fully mixed, fermented and cultivated at 28°C for 10 days, and the solid medium is covered with w...

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Abstract

The invention discloses a preparation method and applications of a fusarium oxysporum inoculant. The inoculant is prepared by the following steps: taking fusarium oxysporum as the mother inoculant, inoculating a fermentation liquid containing fusarium oxysporum to a solid culture medium to carry out solid fermentation, and fully and evenly mixing the fermentation products with diatomite and fosthiazate particles so as to obtain the inoculant. The inoculant has the advantages that: fusarium oxysporum can colonize in plants, but does not cause diseases, is an internal bio-control bacterium, and has a unique advantage on preventing and treating root-knot nematode; furthermore, because of the endoparasitism characteristic, fusarium oxysporum is seldom affected by competitions from other external micro-organisms, thus the preventive effect can be stably exerted; fusarium oxysporum can be directly used to prevent and treat root-knot nematode and cooperatively used with fosthiazate, can effectively control the harms caused by root-knot nematode, at the same time prominently reduces the usage amount of fosthiazate, promotes the crop growth, and realizes high yield, safe, and harmless vegetable production.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a preparation method and application of a Fusarium oxysporum bacterial agent. Background technique [0002] At present, root-knot nematodes are common in crops such as cucumbers and tomatoes in my country, especially in protected cultivation, and the annual losses to various crops reach billions of dollars. After root-knot nematode infestation, root knots are formed on the roots of the crops, and the absorption function of the roots is destroyed, resulting in dwarfing, yellowing and even leaf fall off of the aboveground parts. [0003] Due to the scarcity of root-knot nematode-resistant germplasm resources, the important measures for controlling root-knot nematode in related technologies are chemical control, such as methimenphos, potassium bromide and dimucarb. Although these pesticides kill insects quickly, they will seriously reduce the quality of vegetables, pollute the env...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P5/00A01N57/32
Inventor 茆振川朱萍萍凌键陈国华杨宇红谢丙炎
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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