Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method

Abstract

The invention discloses a rooting culture medium for a tissue culture seedling of photinia fraseri as well as an in-bottle rooting method and an outside-bottle rooting method. The rooting culture medium takes a 1 / 2MS culture medium as a basic culture medium and is added with 0.2mg / L of naphthylacetic acid, 0.1mg / L of indol-3-butyric acid, 25g / L of sucrose and 4g / L of agar. The outside-bottle rooting method comprises the following steps: cutting a single bud from subculture cluster buds cultured by the tissue culture seedling of the photinia serrulata and immersing the cut single buds into naphthylacetic acid with the concentration being 500mg / L for 5 seconds; and carrying out cuttage on the single bud so as to insert into a turfy soil base material for rooting culture. According to the rooting culture medium, the in-bottle rooting method and the outside-bottle rooting method, researches on a rooting technology of the tissue culture seedling of the photinia serrulata are carried out on the basis of a developed rapid tissue culture propagation technology of the photinia serrulata; the rooting rate of the rooting culture medium is 100% and the average rooting quantity is 5; the rooting rate of the outside-bottle rooting method is 98.3% and the average rooting quantity is 7.6. The rooting culture medium, the in-bottle rooting method and the ex-bottle rooting method have important practical significances on industrial and large-scale rapid seedling growing of the photinia serrulata.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a rooting medium for Photinia fragrans tissue culture seedlings, a rooting method in a bottle and a rooting method outside a bottle. Background technique [0002] Photinia fraseri (Photinia fraseri) is a hybrid species of Photinia (Photinia) in the Rosaceae family. It is an evergreen small tree named for its bright red shoots and young leaves. As a precious evergreen color-leaved tree species, Photinia fragrans has the advantages of rapid growth, pruning resistance, compact plant shape, strong germination ability, and wide range of suitable growth. , cold-resistant and barren-resistant, it can be planted in most areas south of the Yellow River. The plant has strong resistance to poisonous gas and can be planted in areas with serious air pollution. It is widely used in gardens, and has good effects as hedges, green walls, modeling trees, and isolated plants. As a newl...

AI Technical Summary

Problems solved by technology

Conventional cutting propagation, due to factors such as slow propagation speed, seasonal restrictions, and large footprint, greatly limits its promotion speed

Compared with cutting seedlings, Photinia fragrans tissue-cultured seedlings are not subject to seasonal restrictions and are more suitable for industrial seedlings. However, Photinia fragrans tissue-cultured seedlings have fewer roots, low rooting rate, and low root activity. Research on industrial production and application is relatively poor. few

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