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A method of differentiating oncogenic changes of the thyroid, a kit to embody the method and the use of metallothionein (mt) for differentiating oncogenic changes of the thyroid

A technology of metallothionein and sulfur protein, which is applied in the direction of analyzing materials, biomaterial analysis, measuring devices, etc., and can solve the problems that the predicted value has not been confirmed.

Inactive Publication Date: 2014-02-05
弗罗茨瓦夫研究中心有限公司
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Problems solved by technology

Although the role of metallothionein in various malignancies has been studied for many years, its predictive value in the several thyroid tumors mentioned above has not been confirmed

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  • A method of differentiating oncogenic changes of the thyroid, a kit to embody the method and the use of metallothionein (mt) for differentiating oncogenic changes of the thyroid
  • A method of differentiating oncogenic changes of the thyroid, a kit to embody the method and the use of metallothionein (mt) for differentiating oncogenic changes of the thyroid
  • A method of differentiating oncogenic changes of the thyroid, a kit to embody the method and the use of metallothionein (mt) for differentiating oncogenic changes of the thyroid

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[0019] The 186 paraffin blocks of various thyroid lesions used in this study were all from the Department of Pathomorphology, Wroclaw Medical Academy. The paraffin blocks examined included 92 cancers, including 48 papillary carcinomas, 35 follicular carcinomas, 9 medullary carcinomas, 31 adenomas, and 63 neoplastic goiters.

[0020] A. Immunohistochemistry

[0021] Tumor biopsies were fixed in 10% buffered formalin, dehydrated, and embedded in paraffin blocks. All immunohistochemical (IHC) reactions were performed on 4 μm thick paraffin sections, deparaffinized in xylene, and rehydrated. For immunohistochemical anti-Ki-67 reactions, sections were incubated in citrate buffer (pH 6.10 mM) at 95-98°C for 20 minutes to expose the epitope. Put it in 3%H 2 o 2 Incubate for 5 minutes to block endogenous peroxidase activity. Expression of MT I / II (clone E9) and Ki-67 (clone MIB-1) was determined using mouse monoclonal antibodies (DakoCytomation, Glostrup, Denmark). The detected ...

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Abstract

A method of differentiating oncogenic changes of the thyroid encompassing follicular adenoma and follicular cancer, characterised in that in a sample of oncogenic tissue from a patient the expression of the metallothionein (MT) potein is evaluated using immunochistochemical methods using monoclonal anti-MT antibodies, the degree of intensity of the expression of metallothionein is evaluated under a light microscope using the semiquantitative IRS method according to Remmele, wherein if the degree of intensity of the expression of metallothionein is equal to or greater than 8 on the semiquantitative IRS scale then the presence of follicular cancer is indicated.

Description

technical field [0001] The subject of the present invention is a method for differentiating thyroid carcinogenesis comprising follicular adenoma and follicular carcinoma, a kit for carrying out this method and the use of metallothionein for differentiating the aforementioned types of thyroid carcinogenesis. The subject of the present invention is the field of diagnosis and of the differentiation of thyroid tumors by immunohistochemical methods. Background technique [0002] Thyroid cancer is the most common endocrine system tumor. Follicular cells of the thyroid develop into well-differentiated cancers (papillary and follicular) that are common in younger people and poorly differentiated degenerative cancers that are common in people over sixty years of age. Papillary carcinoma accounts for 70-80% of thyroid cancers and is considered the mildest form of cancer in this organ. Follicular carcinoma accounts for 5-15% of thyroid cancers. Follicular carcinoma and follicular ad...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577
CPCG01N2333/825G01N33/6893G01N33/57407
Inventor 皮奥特·德兹艾基尔玛兹纳·博得尔斯卡奥克罗安娜·克罗里卡克里斯托弗·克比尔斯基巴托斯·普拉艾里克桑德拉·皮奥托斯卡
Owner 弗罗茨瓦夫研究中心有限公司
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