Primer and kit for detecting microdeletion of chromosome Y and use method of primer

A Y chromosome and kit technology, applied in the field of molecular biology, can solve the problems of complicated hybridization operation, low electrophoresis sensitivity and low accuracy, and achieve the effects of high sample throughput, high detection sensitivity and reduced detection cost.

Active Publication Date: 2014-02-12
SHANGHAI SIMPLEGENE CLINICAL LAB CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Currently, the main method for detection of Y chromosome microdeletions is to specifically amplify the sequence tag site (STS) in the AZF region of the Y chromosome by PCR, and then detect the PCR amplification products by electrophoresis or Southern blot hybridization, but the sensitivity of electrophoresis is low, The accuracy is not high, the hybridization operation is cumbersome, time-consuming and labor-intensive, and the cost is high

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  • Primer and kit for detecting microdeletion of chromosome Y and use method of primer
  • Primer and kit for detecting microdeletion of chromosome Y and use method of primer
  • Primer and kit for detecting microdeletion of chromosome Y and use method of primer

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Embodiment

[0047] Kit for detecting Y chromosome microdeletion and use thereof

[0048] 1. The kit for detecting Y chromosome microdeletions contains:

[0049] ⑴ Primers

[0050]The primer sequences for specific amplification of the SY84 site are shown in SEQ ID NO: 1 and SEQ ID NO: 2,

[0051] SEQ ID NO: 15'-AGAGGAGTCCTGATGCAGGT-3'

[0052] SEQ ID NO:25'-GCCCATTACCTGGAGCCTTC-3'

[0053] The primer sequences for specific amplification of the SY86 site are shown in SEQ ID NO:3 and SEQ ID NO:4,

[0054] SEQ ID NO:35'-GTGAACCACAGACATGCTTC-3'

[0055] SEQ ID NO: 45'-ACACACAAGGCGAACCCCT-3'

[0056] The primer sequences for specifically amplifying the SY127 site are shown in SEQ ID NO:5 and SEQ ID NO:6,

[0057] SEQ ID NO:55'-GGCTCTCACACGAATAGAAA-3'

[0058] SEQ ID NO:65'-CTGCTGGCACTAATATGGGA-3'

[0059] The primer sequences for specifically amplifying the SY134 site are shown in SEQ ID NO:7 and SEQ ID NO:8,

[0060] SEQ ID NO:75'-GTCTCGCTCACACTAAAACG-3'

[0061] SEQ ID NO:85'-ACCAAC...

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Abstract

The invention discloses a primer and a kit for detecting microdeletion of a chromosome Y and a use method of the primer. The primer disclosed by the invention is a specific primer aiming at six STSs (Sequence Tagged Sites) and reference genes, and the kit containing the primer can be used for rapidly and accurately detecting the microdeletion of the chromosome Y through the combination of multiplex PCR (Polymerase Chain Reaction) amplification and DHPLC (Denaturing High Performance Liquid Chromatography) detection, so that detection program is simple and the detection sensitivity, the result repeatability and the sample flux are high.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, relates to a primer and a kit for gene detection, in particular to a primer and a kit for detecting a Y chromosome microdeletion and a method for using the same Background technique [0002] It is estimated that about 10% of couples of childbearing age in the world are infertile, of which reproductive disorders caused by male factors account for about half, and the proportion of male infertility caused by genetic factors is 30%, of which the two with the highest incidence rate are Klinefelter syndrome 10-15% of azoospermia or oligospermia have Y chromosome microdeletion. [0003] The Y chromosome is the shortest apex centromere chromosome and is a male-specific chromosome. There are multiple genes involved in the spermatogenesis process on the Y chromosome. Studies have shown that there is a key fertility-related factor region on the Y chromosome, called Azoospermia factor (AZF), it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2537/143C12Q2565/137C12Q2545/101
Inventor 胡广庆张涵刘晓峰侯然梁超王绪华方国伟黄士昂陈忠
Owner SHANGHAI SIMPLEGENE CLINICAL LAB CO LTD
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