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Method for screening related genes of fruit fly intestinal immunity

An immune-related, Drosophila technology, applied in animal husbandry and other fields, can solve the problems of undiscovered, unexplained regulatory factors of signaling pathways, unclear host meanings, etc.

Inactive Publication Date: 2014-02-26
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the Drosophila model has provided a lot of information for the study of intestinal immunity in recent years, some important issues still remain to be resolved. The significance of the NF-κB activity and the basic expression of ROS induced by the commensal flora to the host is still unclear, and the regulators of some important signaling pathways have not yet been discovered

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  • Method for screening related genes of fruit fly intestinal immunity
  • Method for screening related genes of fruit fly intestinal immunity
  • Method for screening related genes of fruit fly intestinal immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening of genes that affect the intestinal resistance of Drosophila to microbial infection

[0034] In the previous experiments, the inventor screened 15 genes that play an important role in regulating the innate immune response caused by fungal infection, and the functions of these genes in the intestinal immunity of Drosophila have not yet been reported. The fungal infection genes were the research objects, and the detailed gene names and their P factor insertion-deletion mutants are shown in Table 1. In this experiment, wild-type Drosophila W 1118 as the control group (WT).

[0035] Table 1: P factor insertion gene deletion mutants

[0036]

[0037]

[0038] 1. Effects of feeding B. bassiana spores and M. luteus on the survival rate of wild-type and mutant Drosophila

[0039] The experimental process was as follows: wild-type and mutant fruit flies that had been eclosion for 3-4 days were collected, and 15 female and 15 male fruit flies were se...

Embodiment 2

[0048] Example 2 Screening genes that can affect the intestinal stress response of Drosophila

[0049] In this example, the inventors used the 15 anti-fungal infection gene mutant Drosophila described in Example 1 as screening objects, and selected 3 typical and representative strains in the study of Drosophila intestinal stress response. stimuli, where H 2 o 2 It can induce intestinal oxidative stress, while SDS (surfactant, toxic chemical reagent) and NaCl (high salt stress) can stimulate intestinal inflammation.

[0050] 1. Feed H 2 o 2 , SDS and NaCl on the survival rate of wild-type and mutant Drosophila

[0051] The experimental process is as follows: collect wild-type and mutant fruit flies that have been eclosion for 3-4 days, select 15 female and 15 male fruit flies in each group, transfer them to empty fruit fly tubes for starvation treatment for two hours, and then transfer them to the prepared In the SDS, NaCl medium or standard medium, the medium was changed ...

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Abstract

The invention relates to a method for preliminarily screening related genes of fruit fly intestinal immunity. According to the method, after feeding gene mutant fruit flies with different kinds of microorganisms, peroxides, high-salt ions and chemical substances with high toxicity, survival rates of the mutant fruit flies are observed, intestinal morphology changes and cell apoptosis of the fruit flies low in survival rate are detected, activation of some known signal channels participating in the intestinal immune in the intestinal tracts of the mutant fruit flies is detected through a real-time polymerase chain reaction (PCR) method, therefore, the genes related to the fruit fly intestinal immunity can be preliminarily screened systematically, easily and quickly, researchers can know complex intestinal immunity regulatory mechanism, and a new idea for treating intestinal diseases is provided.

Description

technical field [0001] The invention belongs to the field of natural immunity of Drosophila, and relates to a method for screening genes related to intestinal immunity of Drosophila. Background technique [0002] In addition to the function of digestion and absorption, the intestinal mucosa is also a barrier between the internal and external environment, which can protect the host from the invasion of pathogenic microorganisms, and is also a regulator of the flora and the host immune system. When constantly stimulated by the external environment, intestinal epithelial cells respond to internal and external factors to ensure their own survival and proper intestinal environment. In recent years, more and more people are troubled by intestinal inflammatory diseases, and prolonged inflammation and tissue damage will lead to intestinal cancer and tumor formation. Although the human body can resist tumors through natural and acquired immunity, in reality, due to the existence of ...

Claims

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Application Information

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IPC IPC(8): A01K67/033C12Q1/68
Inventor 金丽华郝阳光刘强
Owner NORTHEAST FORESTRY UNIVERSITY
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