Verification method for inhibiting saliva flora odor production by natural extract

A technology of natural extracts and verification methods, which is applied in the field of verification of natural extracts inhibiting saliva bacteria to produce odor, can solve the problems of lack of integrity, cost of manpower and financial resources, and inapplicability of preliminary screening, etc., and achieve simplified verification methods and expanded verification Scope, the effect of increasing the integrity of the experiment

Pending Publication Date: 2022-03-01
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the inhibition zone test can only verify the inhibitory effect of natural extracts on some specific strains related to halitosis, which lacks integrity; Consuming manpower and financial resources is not suitable for the laboratory's initial screening of natural extracts that can improve bad breath

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] 1) Sample preparation: use the saliva collected from the oral cavity of patients with halitosis or related bacterial strains that cause halitosis as raw materials to prepare a bacterial suspension with a certain concentration for use;

[0031] 2) Preparation of natural extracts: extract natural products to obtain natural extracts;

[0032] 3) Collect the gas produced by the saliva flora: take 9ml of the sample from step 1) and put it in a centrifuge tube, add 1ml of the natural extract prepared in step 2), mix well, put it into a Duchenne tubule, and place it in a 37°C constant temperature incubator Cultivate for 1-7 days to obtain a test sample; use the sample in step 1) as a positive control, and use sterile water or a sterile liquid medium as a negative control;

[0033] 4) Test the content of volatile sulfur-containing compounds in the generated gas: take out the test sample, positive control and negative control after step 3) cultivation, use a syringe to extract t...

Embodiment 1

[0035] 1. Saliva sample: collect the saliva of halitosis patients, fasting for 1 hour before collecting water, dilute the collected saliva with sterile water within 0.5 hour to a concentration of 2×10 6 CFU / ml spare.

[0036] 2. Preparation of bacterial suspension: use anaerobic liquid medium or nutrient broth medium to activate the test strain (Klebsiella, stored in the laboratory); take fresh culture, use anaerobic liquid medium or nutrient broth The culture medium was prepared at a concentration of 2×10 6 CFU / ml bacterial suspension.

[0037] 3. Preparation of natural extract: the natural extract (Prunella vulgaris) was extracted by ultrasonic, using 80% ethanol as the extraction solvent, and ultrasonically extracted for 40 minutes; it was used as a test sample for later use.

[0038] 4. Collect saliva flora to produce gas:

[0039] Take 9ml concentration as 2×10 6 Put the CFU / ml saliva sample or bacterial suspension in a 50ml centrifuge tube, then add 1ml of the prepar...

Embodiment 2

[0045] This Example 2 is similar to Example 1, except that the natural extract is replaced by Prunella vulgaris extract by Prunella vulgaris extract, and the results show that Prunella vulgaris is the same as that of Prunella vulgaris, and the VSCs content of the extract is lower than that of the positive sample , has an inhibitory effect.

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PUM

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Abstract

The invention discloses a verification method for inhibiting saliva flora odor production by a natural extract, and the method comprises the following steps: 1) preparing a sample: preparing a bacterial suspension with a certain concentration by taking saliva collected from the oral cavity of a halitosis patient or related strains causing halitosis as a raw material; (2) preparing a natural extract; (3) collecting generated gas, namely putting the sample in the step (1) into a centrifugal tube, adding the natural extract prepared in the step (2), uniformly mixing, putting into a Dunaliella tubule, and culturing in a constant-temperature incubator; and 4) testing the content of the volatile sulfur-containing compounds in the generated gas: taking out the sample cultured in the step 3) and the contrast, extracting the collected gas, and injecting the gas into a gas chromatograph to analyze and judge the content of the volatile sulfur-containing compounds in the gas. According to the application, the verification range of the natural extract for inhibiting the odor production of the saliva flora is expanded; the verification method is simplified, so that the method is more suitable for screening natural extracts for inhibiting odor production of saliva flora in a laboratory.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for verifying that natural extracts inhibit salivary flora from producing odor. Background technique [0002] Bad breath is the bad smell that comes out when you breathe. The gases that cause bad breath mainly include volatile sulfur compounds (VSCs), such as hydrogen sulfide, methyl mercaptan and dimethyl sulfide, in addition to volatile aromatic hydrocarbon compounds, such as indole, methyl indole, organic acids Such as acetic acid, propionic acid, amines such as cadaverine, putrescine and other organic substances can also cause bad breath. Some studies have shown that the types of flora microorganisms and the number of aerobic and anaerobic bacteria in the oral cavity of patients with halitosis are higher than those of normal people. Common bacterial strains that cause halitosis include Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella Bacteria, Klebsiella, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCC12Q1/025C12Q2304/40
Inventor 高茜李雪梅熊文米其利罗娜蔡炳彪蒋佳芮许力
Owner CHINA TOBACCO YUNNAN IND
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