Method for obtaining and cultivating sphagnum protonema

A culturing method and a sphagnum moss technology are applied in the field of natural resource utilization and protection, and can solve the problems of difficulty in collecting sphagnum spores, short protonema growth cycle, and lack of stable cultivation of sphagnum sphagnum protonema, and the like. The effect of protecting the ecological environment and improving the application efficiency

Active Publication Date: 2014-03-05
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difficulty in collecting sphagnum spores, and the short growth cycle of the protonema, it will rapidly differentiate into stem and leaf bodies within a few days. At present, there is no report on the stable mass cultivation of sphagnum protonema

Method used

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  • Method for obtaining and cultivating sphagnum protonema
  • Method for obtaining and cultivating sphagnum protonema
  • Method for obtaining and cultivating sphagnum protonema

Examples

Experimental program
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Embodiment 1

[0023] The culture method of embodiment 1 peat moss protonema

[0024] Raw material: stems and leaves of peat moss (Sphagnum squarrosum) collected from Dagu Glacier in Sichuan, my country.

[0025] The formula of the improved Knop liquid medium is: Potassium dihydrogen phosphate (KH 2 PO 4 ) 250 mg / L, Potassium Chloride (KCl) 250 mg / L, Magnesium Sulfate (MgSO 4 .7H 2 O) 250 mg / L, calcium nitrate (Ca(NO 3 ) 2 .4H 2 O) 1000 mg / L, ferrous sulfate (FeSO 4 .7H 2 O) 14 mg / L, pH 5.4.

[0026] Equipment for continuous tissue crushing: pulverizer (Fluko Germany, FA25).

[0027] Specific steps: Cultivate peat moss for 2 weeks under moist soil conditions, and collect newly grown stems and leaves for detoxification. The detoxification method is 70% ethanol for 30 seconds, rinsed with sterile water for 3-5 times, then dry the stem and leaf bodies with sterile filter paper, and finally place them on the improved Knop solid medium for cultivation to obtain sterile Stem material. ...

Embodiment 2

[0028] Embodiment 2 utilizes the sphagnum protonema of cultivation to propagate the stem and leaf body of sphagnum moss

[0029] Raw material: the aseptic sphagnum moss protonema obtained by culturing in Example 1

[0030] The formula of the improved Knop liquid medium is: Potassium dihydrogen phosphate (KH 2 PO 4 ) 250 mg / L, Potassium Chloride (KCl) 250 mg / L, Magnesium Sulfate (MgSO 4 .7H 2 O) 250 mg / L, calcium nitrate (Ca(NO 3 ) 2 .4H 2 O) 1000 mg / L, ferrous sulfate (FeSO 4 .7H 2 O) 14 mg / L, pH 5.4.

[0031] The formula of the modified solid Knop medium is: liquid modified Knop+0.8% agar.

[0032] Culture conditions: temperature 18-22°C, shaker speed 120 rpm, 24h shaking, medium pH 5.4, suspension culture, light 3000Lux, light time 16h light / 8h dark.

[0033] Specific steps: stop the continuous tissue crushing of the sphagnum protonema cultivated in the above-mentioned embodiment 1, and place it on a shaker for culture, and the stem and leaf body can grow in 10 day...

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Abstract

The invention provides a method for obtaining and cultivating sphagnum protonema. The method comprises the following steps: carrying out tissue smashing on sphagnum cormus used as a raw material; cultivating in a liquid culture medium; growing new protonema from fragments of sphagnum tissue cormus, and carrying out tissue smashing on the protonema; carrying out liquid cultivation, so as to obtain the sphagnum protonema. By adopting the method, a lot of protonemata of the sphagnum can be obtained; the cormus with uniform growth can be largely obtained by using the protonema within a short period of time; application of the sphagnum in a tissue culture physiological experiment and sphagnum protoplast preparation, transformation or regeneration is facilitated.

Description

technical field [0001] The invention relates to the field of utilization and protection of natural resources, in particular to a method for obtaining and cultivating sphagnum moss protoceles. Background technique [0002] The genus Sphagnum belongs to the phylum Bryophyta, the class Moss, and the order Sphagnum. This order has only one family and one genus. There are more than 300 species of peat moss in the world, and more than 40 species in my country. Peat moss generally grows in swampy areas, and grows luxuriantly in the swampy areas of the northeast and southwest of my country. Peat moss is one of the important charcoal-making plants. It will accumulate a large amount of peat during the long-term growth process, and will eventually become a peat deposit with industrial value. The carbon sequestration of peat moss accounts for half of the total carbon sequestration of peat bogs in the northern hemisphere. . In addition, peat moss is one of the main plant groups in wet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙越李泽玲李小方朱瑞良
Owner EAST CHINA NORMAL UNIV
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