Application of codiaceae polysaccharide

The technology of pine algae polysaccharide and pine algae is applied in the application field of pine algae polysaccharide, which can solve the problem that the anti-complement activity of pine algae polysaccharide has not been studied, and achieve the effect of inhibiting excessive activation.

Active Publication Date: 2014-03-12
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A small amount of literature has reported that SCEC has biological activities such as anticoagulation and antioxidation, but the anti-complement activity of SCEC has not yet been studied

Method used

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  • Application of codiaceae polysaccharide
  • Application of codiaceae polysaccharide
  • Application of codiaceae polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, preparation of spinosa polysaccharide (CF)

[0024] Take 50 g of dry pine algae, use 2000 mL of tap water to extract in a pressure cooker at 115 °C for 3 hours, filter the algae residue through a sieve, filter with diatomaceous earth, filter the extract, concentrate the filtrate and put it into a Dialysis in a dialysis bag, 48 hours of tap water dialysis, 24 hours of distilled water dialysis, concentrated to a small volume and then freeze-dried to obtain the crude product of echinococcus polysaccharide (CF) extracted by water, with a yield of 2.10%.

[0025] The results of chemical composition analysis, monosaccharide ratio analysis, sulfate content and molecular weight determination of CF are shown in Table 1. The results of the IR spectrum show that P. chinensis is a sulfated polysaccharide, at 1242 cm -1 and 845 cm -1 The characteristic absorption peaks of sulfuric acid groups appear on the left and right sides.

[0026] Table 1. Chemical analysis o...

Embodiment 2

[0028] Example 2, the role of anti-complement activity of the classic pathway of CF samples in Example 1

[0029] In this test, the anti-complement activity of the classical pathway is used to reflect the effect of the sample on complement, and to illustrate its role in anti-complement activity.

[0030] Test sample: spinosa polysaccharide CF in embodiment 1

[0031] Test method: Add 100 μL of pH 7.0-7.5 sample solution prepared with phosphate-buffered saline into the test tube; then add 100 μL of normal guinea pig serum diluted 1:100 with glucose-gelatin barbiturate buffered saline and 100 μL of sensitized Sheep red blood cells (5×10 8 samples / mL), mix gently; incubate in a water bath at 37°C for 30 min, shake gently from time to time, and then add 1 mL of cold normal saline to terminate the reaction; centrifuge at 2000 r / min for 10 min, and take the supernatant in The light absorption value was measured at a wavelength of 405 nm; the inhibition rate of the sample on the h...

Embodiment 3， Embodiment 1

[0033] Example 3, the role of anti-complement activity of the alternative pathway of CF samples in Example 1

[0034] This test uses the anti-complement activity of the alternative pathway to reflect the effect of the sample on complement, to illustrate its role in anti-complement activity.

[0035] Test sample: spinosa polysaccharide CF in embodiment 1

[0036] Test method: Take 100 μL of pH 7.0-7.5 sample solution prepared with phosphate-buffered saline and add it to the test tube; then add an equal volume of GVB-Mg-EGTA (2×) buffer solution (containing 5 mMC barbital sodium, mol / L NaCl, 0.1% gelatin, 4 mmol / L magnesium chloride, 16mmol / L EGTA, pH 7.4, normal guinea pig serum diluted 1:2 and 100 μL rabbit red blood cell suspension (1.5×10 8samples / mL), mix gently; incubate in a water bath at 37°C for 30 min, shake gently from time to time, and then add 1 mL of cold normal saline to terminate the reaction; centrifuge at 2000 r / min for 10 min, and take the supernatant in The...

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Abstract

The invention provides application of codiaceae polysaccharide to an anti-complement drug. The codiaceae polysaccharide has complement activity, and the application of the codiaceae polysaccharide refers to the application of the codiaceae polysaccharide with the complement activity to the preparation of the anti-complement drug or/and an anti-complement health-care product. The invention discovers the remarkable inhibiting function of the codiaceae polysaccharide on the classical pathway complement activity and the alternative pathway complement activity for the first time and discloses the application of the codiaceae polysaccharide to the preparation of the anti-complement drug and the anti-complement health-care product, so that the excessive activation of a complement system can be inhibited and further the certain preventive and therapeutic effects on various diseases such as systemic lupus erythematosus, rheumatoid arthritis and acute respiratory distress syndrome caused by the excessive activation of the complement system are achieved.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of chaeose polysaccharide with complement activity. Background technique [0002] The complement system is one of the important immune defense systems of the human body. However, the abnormal activation of this system will cause the excessive response of the human immune system and cause damage to the normal tissues of the human body. Studies have shown that excessive activation of complement is involved in the pathological process of various diseases, such as rheumatoid arthritis, senile dementia, systemic lupus erythematosus, rejection after organ transplantation, multiple organ failure syndrome, acute myocardial infarction, acute Respiratory Distress Syndrome (ARDS), etc. At present, there is no ideal drug for the treatment of such diseases. At the same time, in view of the important role of complement overactivation in causing many critical human diseases, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A23L1/29A61P37/02A61P37/06A61P29/00A61P19/02A61P11/00A23L33/00
Inventor 张全斌王晶张虹牛锡珍
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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