Hyaluronic acid-orientated channel composite bracket material used for spinal cord injury repair

Abstract

The invention discloses a hyaluronic acid- orientated channel composite bracket material used for spinal cord injury repair. The hyaluronic acid- orientated channel composite bracket material is composed of a hydrogel antibody releaser grafted with an NoGoR antibody and a factor-packaging poly lactic-co-glycolic acid copolymer microsphere which is injected into the hydrogel antibody releaser, wherein the inner part of the hydrogel antibody releaser grafted with the NoGoR antibody is shaped like a longitudinal channel which is in parallel arranged; the factor-packaging poly lactic-co-glycolic acid copolymer microsphere is shaped like a circular ball, and smooth in surface; the factor packaged by the polylactic acid-polyglycolic acid copolymer microsphere is a vascular endothelial growth factor and a brain-derived neurotrophic factor. The bracket material disclosed by the invention can improve a nerve regeneration microenvironment to promote spinal cord injury repair.

Description

technical field

[0001] The invention relates to a hyaluronic acid directional channel composite scaffold material, in particular to a hyaluronic acid directional channel composite scaffold material for spinal cord injury repair.

[0002] The present invention also relates to a preparation method of the above-mentioned hyaluronic acid directional channel composite scaffold material.

[0003] The present invention also relates to the application of the above-mentioned hyaluronic acid directional channel composite scaffold material. Background technique

[0004] Spinal cord injury is mainly caused by trauma, mostly in young adults. The injury causes the death of local neurons in the spinal cord, the rupture of the ascending and descending fiber bundles, and the sensory, motor, and visceral dysfunction below the level of injury, leading to varying degrees of hemiplegia or paraplegia, and many patients are permanently disabled. At present, clinical treatment is mainly palliativ...

Examples

preparation example Construction

[0054] The method for preparing a hyaluronic acid directional channel composite scaffold material for spinal cord injury repair provided by the present invention, first prepares HA and PLL into a hydrogel with longitudinal channels, uses the oxidation grafting method to connect NogoR antibodies, and prepares the encapsulating BDNF and The PLGA microspheres of VEGF were injected into the HA material before transplantation to obtain a hyaluronic acid-oriented channel composite scaffold material for spinal cord injury repair.

[0055] Specifically, its main steps are:

[0056] 1) Take 0.20g of sodium hyaluronic acid, add 20ml of deionized water to make a 1% hyaluronic acid solution, stir well; add 0.04g of polylysine (PLL), mix well and add adipic acid di Hydrazide (ADH) 1.5g, stir well; add 1mol / L HCl (about 3ml) dropwise until the pH value reaches 4-5; then add carbodiimide salt (EDC) 0.4g, stir well for 10min-20min, The solution gradually turns into a gel; use 1mol / L NaOH to ...

Embodiment 1

[0063] 1) Preparation of hyaluronic acid hydrogel oriented channel scaffold

[0064] According to relevant literature, take 0.20g of sodium hyaluronic acid, add 20ml of deionized water, and make a 1% hyaluronic acid solution, stir well; add 0.04g of polylysine (PLL), mix well Acid dihydrazide (ADH) 1.5g, stir well; add 1mol / L HCl (about 3ml) dropwise until the pH value reaches 4~5; then add carbodiimide salt (EDC) 0.4g, stir well for 10min~ After 20 minutes, the solution gradually turned into a gel; the pH value was adjusted to 7 with 1mol / L NaOH, and then the fluid was injected into a known mold, the upper and lower ends were opened, and the surroundings were wrapped with foam plastics, immersed in liquid nitrogen and frozen for 30 minutes; Freeze for 2 hours and dry in a freeze vacuum dryer for 48 hours to shape.

[0065] 2) Hyaluronic acid hydrogel oriented channel scaffold grafted with NogoR antibody

[0066] Add sodium periodate to the NogoR antibody PBS solution at a c...

Embodiment 2

[0078] Effects of Hyaluronic Acid Composite Scaffold on Neurons in Vitro

[0079] Rat cortical neurons were inoculated on the hyaluronic acid composite scaffold material prepared in Example 1, and neurons were also inoculated on ordinary hyaluronic acid hydrogel, and NF immunofluorescent staining after culturing for 1 week and counted 10 randomly The number of NF-positive cells in the field of view was statistically found that the number of NF-positive cells inoculated on the hyaluronic acid composite scaffold material was significantly more than the number of cells inoculated on the ordinary hyaluronic acid hydrogel, indicating that the hyaluronic acid composite scaffold material can promote neurogenesis. meta-adhesion, growth (see Figure 6 ).

technical field

[0001] The invention relates to a hyaluronic acid directional channel composite scaffold material, in particular to a hyaluronic acid directional channel composite scaffold material for spinal cord injury repair.

[0002] The present invention also relates to a preparation method of the above-mentioned hyaluronic acid directional channel composite scaffold material.

[0003] The present invention also relates to the application of the above-mentioned hyaluronic acid directional channel composite scaffold material. Background technique

[0004] Spinal cord injury is mainly caused by trauma, mostly in young adults. The injury causes the death of local neurons in the spinal cord, the rupture of the ascending and descending fiber bundles, and the sensory, motor, and visceral dysfunction below the level of injury, leading to varying degrees of hemiplegia or paraplegia, and many patients are permanently disabled. At present, clinical treatment is mainly palliativ...

Examples

preparation example Construction

[0054] The method for preparing a hyaluronic acid directional channel composite scaffold material for spinal cord injury repair provided by the present invention, first prepares HA and PLL into a hydrogel with longitudinal channels, uses the oxidation grafting method to connect NogoR antibodies, and prepares the encapsulating BDNF and The PLGA microspheres of VEGF were injected into the HA material before transplantation to obtain a hyaluronic acid-oriented channel composite scaffold material for spinal cord injury repair.

[0055] Specifically, its main steps are:

[0056] 1) Take 0.20g of sodium hyaluronic acid, add 20ml of deionized water to make a 1% hyaluronic acid solution, stir well; add 0.04g of polylysine (PLL), mix well and add adipic acid di Hydrazide (ADH) 1.5g, stir well; add 1mol / L HCl (about 3ml) dropwise until the pH value reaches 4-5; then add carbodiimide salt (EDC) 0.4g, stir well for 10min-20min, The solution gradually turns into a gel; use 1mol / L NaOH to ...

Embodiment 1

[0063] 1) Preparation of hyaluronic acid hydrogel oriented channel scaffold

[0064] According to relevant literature, take 0.20g of sodium hyaluronic acid, add 20ml of deionized water, and make a 1% hyaluronic acid solution, stir well; add 0.04g of polylysine (PLL), mix well Acid dihydrazide (ADH) 1.5g, stir well; add 1mol / L HCl (about 3ml) dropwise until the pH value reaches 4~5; then add carbodiimide salt (EDC) 0.4g, stir well for 10min~ After 20 minutes, the solution gradually turned into a gel; the pH value was adjusted to 7 with 1mol / L NaOH, and then the fluid was injected into a known mold, the upper and lower ends were opened, and the surroundings were wrapped with foam plastics, immersed in liquid nitrogen and frozen for 30 minutes; Freeze for 2 hours and dry in a freeze vacuum dryer for 48 hours to shape.

[0065] 2) Hyaluronic acid hydrogel oriented channel scaffold grafted with NogoR antibody

[0066] Add sodium periodate to the NogoR antibody PBS solution at a c...

Embodiment 2

[0078] Effects of Hyaluronic Acid Composite Scaffold on Neurons in Vitro

[0079] Rat cortical neurons were inoculated on the hyaluronic acid composite scaffold material prepared in Example 1, and neurons were also inoculated on ordinary hyaluronic acid hydrogel, and NF immunofluorescent staining after culturing for 1 week and counted 10 randomly The number of NF-positive cells in the field of view was statistically found that the number of NF-positive cells inoculated on the hyaluronic acid composite scaffold material was significantly more than the number of cells inoculated on the ordinary hyaluronic acid hydrogel, indicating that the hyaluronic acid composite scaffold material can promote neurogenesis. meta-adhesion, growth (see Figure 6 ).

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