Aureobasidium pullulans and application thereof

A technique of Aureobasidium pullulans and filtrate, which is applied in the field of ecology, can solve problems such as pollution and impact on sustainable agricultural development, and achieve low-cost effects

Inactive Publication Date: 2014-03-19
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as the increase in the population of resistant weeds, soil compaction and water pollution, etc., have seriously affected the sustainable development of agriculture.

Method used

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  • Aureobasidium pullulans and application thereof
  • Aureobasidium pullulans and application thereof
  • Aureobasidium pullulans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Experimental materials and methods

[0020] 1.1 Collection of disease samples and isolation and purification of pathogenic bacteria

[0021] The diseased poplar leaves were collected from Ping'an County, Qinghai Province, and the samples were packed in ziplock bags. The symptoms of the disease were recorded and photos were collected in the laboratory, and they were stored in a 4°C refrigerator for later use. The isolation and purification method refers to the isolation method of pathogenic fungi in the plant disease research method of Fang Zhongda (1998), and isolates and cultures the diseased samples of poplar leaves. That is, cut the tissue at the junction of poplar leaf disease and health, put it in 3% sodium hypochlorite for surface disinfection for 3 minutes, then rinse it in sterile water for 3 times, dry the surface water of the tissue with sterile absorbent paper, and place it on a PDA medium plate Incubate in the dark at 25°C. Five tissue blocks were place...

Embodiment 2

[0056] According to the method described in Example 1, the fermentation filtrate was prepared, and the outdoor preliminary test of Aureobasidium pullulans PA-2 for controlling weeds in rapeseed fields was tested. The outdoor experiment was carried out in the experimental field of the Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences. The weeds are mainly wild oats (3-4 leaf stage), pigtail, and quinoa (3-5 leaf wheel stage). The area of ​​the plot is about 4m 2 , spraying amount 200ml, repeated 3 times, 20 days and 40 days after spraying, the control effect and fresh weight effect of wild oats and broad-leaved weeds were investigated. The results are shown in Table 3.

[0057] Table 3 Control effect of Aureobasidium pullulans PA-2 on main target weeds in rapeseed field

[0058]

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Abstract

The invention discloses aureobasidium pullulans and an application thereof. The class of the strain is named aureobasidium pullulans, the full name of the strain is aureobasidium pullulans PA-2, the strain is preserved in China General Microbiological Culture Collection Center No.3 building No.1 Beichen West Road Changyang District Beijing China on October 28, 2013, and the preservation serial number is CGMCC No.8413. The aureobasidium pullulans is used for biologically removing broad-leaf weeds such as cleavers, goosefoots, malva verticillata and the like as well as oat grasses in wheat, rape and pea field lands, and is capable of effectively controlling and preventing the broad-leaf weeds and the oat grasses.

Description

technical field [0001] The invention relates to the field of ecology, in particular to a strain of Aureobasidium pullulans and its application. Background technique [0002] Weed control in farmland mainly uses chemical herbicides as the main body to promote the process of agricultural modernization. With the widespread use of chemical herbicides, the negative effects are becoming more and more obvious. For example, the increase of resistant weed populations, soil compaction and water pollution have seriously affected the sustainable development of agriculture. Therefore, the development of broad-spectrum, high-efficiency, low-toxic new herbicides and the development of biological herbicide technology have become a top priority. The emergence of biological herbicides conforms to this development trend. So far, researchers have conducted a lot of research on nearly 100 pathogenic fungi with herbicidal potential on more than 50 kinds of weeds. Among them, fungi with herbic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P13/02C12R1/645
Inventor 郭青云程亮朱海霞李玮翁华魏有海郭良芝
Owner QINGHAI UNIVERSITY
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